Document Detail

The determination of TBA-reactive substances and alkenals in the presence of antioxidants.
MedLine Citation:
PMID:  10344076     Owner:  NLM     Status:  MEDLINE    
This study investigated the inhibitory efficiency of all-rac-alpha-tocopherol, 2,6-ditert-butyl-p-cresole (BHT), and 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carbonic acid (Trolox) on determination of thiobarbituric acid-reactive substances (TBARS) and short-chain alkenals in rat liver homogenates. The concentration of TBARS was measured fluorophotometrically. Aldehydes were determined after derivatization with methylhydrazine by gas chromatography (GLC). The concentrations of alkenals and TBARS in liver homogenates were diminished when antioxidants were present during the sample preparation. It is suggested that in the absence of antioxidants the samples are autoxidized further during the preparative procedures. For the aldehyde determination all-rac-alpha-tocopherol was the most effective antioxidant to reduce the bias due to autoxidation, whereas for TBARS it was Trolox.
S T Balthazary; H P Sallmann; H Fuhrmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta veterinaria Hungarica     Volume:  47     ISSN:  0236-6290     ISO Abbreviation:  Acta Vet. Hung.     Publication Date:  1999  
Date Detail:
Created Date:  1999-06-28     Completed Date:  1999-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8406376     Medline TA:  Acta Vet Hung     Country:  HUNGARY    
Other Details:
Languages:  eng     Pagination:  155-9     Citation Subset:  IM    
Department of Veterinary Physiology, Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, Morogoro, Tanzania.
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MeSH Terms
Aldehydes / analysis*,  metabolism
Antioxidants / pharmacology*
Butylated Hydroxytoluene / pharmacology
Chromans / pharmacology
Chromatography, Gas
Liver / chemistry*,  drug effects,  metabolism
Rats, Wistar
Thiobarbituric Acid Reactive Substances / analysis*,  metabolism
Vitamin E / pharmacology
Reg. No./Substance:
0/Aldehydes; 0/Antioxidants; 0/Chromans; 0/Thiobarbituric Acid Reactive Substances; 128-37-0/Butylated Hydroxytoluene; 1406-18-4/Vitamin E; 56305-04-5/6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid

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