| The determination of TBA-reactive substances and alkenals in the presence of antioxidants. | |
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MedLine Citation:
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PMID: 10344076 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study investigated the inhibitory efficiency of all-rac-alpha-tocopherol, 2,6-ditert-butyl-p-cresole (BHT), and 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carbonic acid (Trolox) on determination of thiobarbituric acid-reactive substances (TBARS) and short-chain alkenals in rat liver homogenates. The concentration of TBARS was measured fluorophotometrically. Aldehydes were determined after derivatization with methylhydrazine by gas chromatography (GLC). The concentrations of alkenals and TBARS in liver homogenates were diminished when antioxidants were present during the sample preparation. It is suggested that in the absence of antioxidants the samples are autoxidized further during the preparative procedures. For the aldehyde determination all-rac-alpha-tocopherol was the most effective antioxidant to reduce the bias due to autoxidation, whereas for TBARS it was Trolox. |
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Authors:
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S T Balthazary; H P Sallmann; H Fuhrmann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta veterinaria Hungarica Volume: 47 ISSN: 0236-6290 ISO Abbreviation: Acta Vet. Hung. Publication Date: 1999 |
Date Detail:
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Created Date: 1999-06-28 Completed Date: 1999-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8406376 Medline TA: Acta Vet Hung Country: HUNGARY |
Other Details:
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Languages: eng Pagination: 155-9 Citation Subset: IM |
Affiliation:
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Department of Veterinary Physiology, Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, Morogoro, Tanzania. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldehydes
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analysis*,
metabolism Animals Antioxidants / pharmacology* Butylated Hydroxytoluene / pharmacology Chromans / pharmacology Chromatography, Gas Female Fluorophotometry Liver / chemistry*, drug effects, metabolism Oxidation-Reduction Rats Rats, Wistar Thiobarbituric Acid Reactive Substances / analysis*, metabolism Vitamin E / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Aldehydes; 0/Antioxidants; 0/Chromans; 0/Thiobarbituric Acid Reactive Substances; 128-37-0/Butylated Hydroxytoluene; 1406-18-4/Vitamin E; 56305-04-5/6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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