Document Detail

The detection of non-O157 E. coli in food by immunomagnetic separation.
MedLine Citation:
PMID:  15186459     Owner:  NLM     Status:  MEDLINE    
AIMS: To compare immunomagnetic separation (IMS) protocols (enrichment media and temperature) for the isolation of Escherichia coli serotypes O26 and O111 from four different foods. METHODS AND RESULTS: Foods (minced beef, cheese, apple juice and pepperoni) spiked with low numbers (<100 g(-1)) of stressed nalidixic mutant E. coli serotypes O26 and O111 were enriched in media based on buffered peptone water (BPW), tryptone soya and EC broths incubated at temperatures of 37 and 42 degrees C to optimize the IMS technique. BPW enrichments gave increased recoveries of both serotypes compared with tryptone soya and EC broths. Elevated temperatures of incubation at 42 degrees C were superior to 37 degrees C. CONCLUSIONS: Positive detection of low numbers of stressed target pathogens in all replicate tests was only possible using BPW enrichments. The majority of tests from alternative enrichments resulted in zero or single colonies recovered post-IMS. SIGNIFICANCE AND IMPACT OF THE STUDY: The optimum IMS protocol would improve isolation rates of E. coli O26 and O111 from foods and lead to increased safety for the consumer. Sub-optimal IMS protocols could lead to foods being incorrectly labelled free from these pathogens.
M Drysdale; M MacRae; N J C Strachan; T M S Reid; I D Ogden
Related Documents :
10932959 - Hazard analysis and critical control points of weaning foods.
15546409 - Growth of enterotoxigenic staphylococcus aureus in povi masima, a traditional pacific i...
9120079 - Rapid and sensitive immunomagnetic separation-polymerase chain reaction method for the ...
10829969 - Microbial contamination of weaning foods.
25006699 - Low-level seaweed supplementation improves iodine status in iodine-insufficient women.
21161279 - Voluntary versus spontaneous swallowing in man.
9930399 - Assessment of the arachidonic acid content in foods commonly consumed in the american d...
6536289 - Alteration of alcohol drinking in the rat by peripherally self-administered acetaldehyde.
18853279 - Saprolegnia brachydanis, a new oomycete isolated from zebra fish.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  97     ISSN:  1364-5072     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2004  
Date Detail:
Created Date:  2004-06-09     Completed Date:  2004-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  220-4     Citation Subset:  IM    
Department of Medical Microbiology, Grampian University Hospital Trust, Aberdeen, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Escherichia coli / isolation & purification*
Food Microbiology*
Immunomagnetic Separation / methods
Sensitivity and Specificity

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Survival of Bacillus cereus spores and vegetative cells in acid media simulating human stomach.
Next Document:  Effect of point-of-use disinfection, flocculation and combined flocculation-disinfection on drinking...