Document Detail


The detection and isolation of protease activity associated with purified preparations of human chorionic gonadotropin.
MedLine Citation:
PMID:  8384996     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The highly purified human CG (hCG) CR series is widely used as a reference material in immunological and biological assays. However these hormone preparations, specifically hCG (CR127), exhibit Arg-specific peptidase activity with synthetic peptide substrates. The putative serine protease-like activity associated with hCG (CR127) was almost completely inhibited by diisopropylfluorophosphate, soybean trypsin inhibitor, N-tosyl-L-phenylalanine chloromethyl ketone and to a lesser extent by N-alpha-p-tosyl-L-lysine chloromethyl ketone and was isolated after hydrophobic interaction and affinity chromatography with soybean trypsin inhibitor, which indicated the presence of exogenous protease contaminants rather than intrinsic peptidase activity. 3H-DFP labeling and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the isolated contaminants revealed two possible serine proteases of apparent mol wts 60,000 and 20,000. The presence of these contaminants had no apparent effect on the receptor binding capability of hCG, however the in vitro biological activity of hCG determined by maximal cAMP production, was decreased after hydrophobic interaction chromatographic purification of the hormone. These observations suggest that the serine protease-like contaminants enhance cAMP production, thereby introducing a significant source of error in biological assays that use hCG (CR127). Further purification of hCG by hydrophobic interaction and affinity chromatography is recommended before its use in bioassays or research.
Authors:
M M Daja; J Hiyama; G K Scott; A G Renwick
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  132     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-04-30     Completed Date:  1993-04-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1766-73     Citation Subset:  AIM; IM    
Affiliation:
Department of Biochemistry, University of Auckland, New Zealand.
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MeSH Terms
Descriptor/Qualifier:
Chorionic Gonadotropin / chemistry*,  isolation & purification
Cyclic AMP / pharmacology
Drug Contamination
Endopeptidases / isolation & purification*,  pharmacology
Humans
Osmolar Concentration
Protease Inhibitors / pharmacology
Radioligand Assay
Receptors, Cell Surface / metabolism
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Protease Inhibitors; 0/Receptors, Cell Surface; 60-92-4/Cyclic AMP; EC 3.4.-/Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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