Document Detail


The detection of human B lymphocytes by both light and electron microscopy utilizing colloidal gold labeled anti-immunoglobulin.
MedLine Citation:
PMID:  344196     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Colloidal gold was used to label rabbit anti-goat IgG for the indirect detection of goat anti-human IgD and IgM. Utilizing this technique, surface immunoglobulin was visualized on human blood B lymphocytes at both the light and electron microscopic levels of resolution. By this technique, percentages of lymphocytes were found to closely correlate with those of the standard immunofluorescent procedure. The ease of the procedure along with the clarity of the marker in both light and electron microscopy suggest wide applicability in clinical and research studies of lymphocyte membrane markers.
Authors:
W D Geoghegan; J J Scillian; G A Ackerman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Immunological communications     Volume:  7     ISSN:  0090-0877     ISO Abbreviation:  Immunol. Commun.     Publication Date:  1978  
Date Detail:
Created Date:  1978-05-17     Completed Date:  1978-05-17     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0353016     Medline TA:  Immunol Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-12     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Anti-Idiotypic*
B-Lymphocytes / ultrastructure*
Colloids
Fluorescent Antibody Technique
Gold / metabolism*
Humans
Immunoglobulin D
Immunoglobulin M
Receptors, Antigen, B-Cell
Chemical
Reg. No./Substance:
0/Antibodies, Anti-Idiotypic; 0/Colloids; 0/Immunoglobulin D; 0/Immunoglobulin M; 0/Receptors, Antigen, B-Cell; 7440-57-5/Gold

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