Document Detail

The detection of cotinine in hydrolyzed meconium samples.
MedLine Citation:
PMID:  10464932     Owner:  NLM     Status:  MEDLINE    
To date, the screening of meconium for the determination of tobacco exposure in newborns has proven difficult. It was hypothesized that cotinine forms reversible Schiff base bonds with free amino functions on proteins, therefore, hydrolysis of meconium would be necessary for the detection of 'free' cotinine. One-hundred-and-two (102) meconium samples received into our laboratory were extracted using a routine non-hydrolysis screening procedure for drugs of abuse. Separate aliquots of the specimens were hydrolyzed and re-extracted according to the same procedure. The results of the two methods were compared using a highly specific cotinine micro-plate enzyme immunoassay procedure (EIA). Of the non-hydrolyzed samples, 33% were positive for cotinine, while 79% of the hydrolyzed samples were cotinine-positive. Common drugs of abuse did not interfere with the analysis. Micro-plate EIA provides a rapid, simple and reliable screening method for the determination of cotinine in meconium following hydrolysis and extraction. In general, the meconium specimens received into our laboratory are from newborns considered to be at risk for post-natal problems due to suspected drug and/or alcohol abuse during pregnancy.
D Dempsey; C Moore; D Deitermann; D Lewis; B Feeley; R S Niedbala
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Forensic science international     Volume:  102     ISSN:  0379-0738     ISO Abbreviation:  Forensic Sci. Int.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-09-09     Completed Date:  1999-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7902034     Medline TA:  Forensic Sci Int     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  167-71     Citation Subset:  IM    
San Francisco Bay Area Regional Poison Control Center, CA, USA.
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MeSH Terms
Cotinine / analysis*
Immunoenzyme Techniques / methods*
Infant, Newborn
Meconium / chemistry*
Neonatal Screening / methods*
Pregnancy Complications / diagnosis,  metabolism
Reproducibility of Results
Schiff Bases
Sensitivity and Specificity
Smoking / metabolism
Substance Abuse Detection / methods*
Reg. No./Substance:
0/Schiff Bases; 486-56-6/Cotinine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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