Document Detail


The detailed analysis of the changes of murine dendritic cells (DCs) induced by thymic peptide: pidotimod(PTD).
MedLine Citation:
PMID:  22863756     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of present research is to analyze the detailed changes of dendritic cells (DCs) induced by pidotimod(PTD). These impacts on DCs of both bone marrow derived DCs and established DC2.4 cell line were assessed with use of conventional scanning electron microscopy (SEM), flow cytometry (FCM), transmission electron microscopy (TEM), cytochemistry assay FITC-dextran, bio-assay and enzyme linked immunosorbent assay (ELISA). We demonstrated the ability of PTD to induce DC phynotypic and functional maturation as evidenced by higher expression of key surface molecules such as MHC II, CD80 and CD86. The functional tests proved the downregulation of ACP inside the DCs, occurred when phagocytosis of DCs decreased, with simultaneously antigen presentation increased toward maturation. Finally, PTD also stimulated production of more cytokine IL-12 and less TNF-α. Therefore it is concluded that PTD can markedly exert positive induction to murine DCs.
Authors:
Xiaofang Hu; Wei Zhang; Lu Wang; Nan Wan; Bing Wang; Weiwei Li; Hui Hua; Xu Hu; Fengping Shan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-06
Journal Detail:
Title:  Human vaccines & immunotherapeutics     Volume:  8     ISSN:  2164-554X     ISO Abbreviation:  Hum Vaccin Immunother     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-12-11     Completed Date:  2013-05-23     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  101572652     Medline TA:  Hum Vaccin Immunother     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1250-8     Citation Subset:  IM    
Affiliation:
Department of Experimental Laboratory, General Hospital of Shenyang Military Command, Shenyang, China. profshan9@163.com
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MeSH Terms
Descriptor/Qualifier:
Adjuvants, Immunologic / pharmacology*
Animals
Antigens, CD80 / metabolism
Antigens, CD86 / metabolism
Dendritic Cells / drug effects*,  metabolism*
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunohistochemistry
Interleukin-12 / metabolism
Mice
Microscopy, Atomic Force
Microscopy, Electron, Transmission
Pyrrolidonecarboxylic Acid / analogs & derivatives*,  pharmacology
Thiazolidines / pharmacology*
Tumor Necrosis Factor-alpha / metabolism
Chemical
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Antigens, CD80; 0/Antigens, CD86; 0/Thiazolidines; 0/Tumor Necrosis Factor-alpha; 187348-17-0/Interleukin-12; 785363R681/pidotimod; 98-79-3/Pyrrolidonecarboxylic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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