| The detailed analysis of the changes of murine dendritic cells (DCs) induced by thymic peptide: pidotimod(PTD). | |
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MedLine Citation:
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PMID: 22863756 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The aim of present research is to analyze the detailed changes of dendritic cells (DCs) induced by pidotimod(PTD). These impacts on DCs of both bone marrow derived DCs and established DC2.4 cell line were assessed with use of conventional scanning electron microscopy (SEM), flow cytometry (FCM), transmission electron microscopy (TEM), cytochemistry assay FITC-dextran, bio-assay and enzyme linked immunosorbent assay (ELISA). We demonstrated the ability of PTD to induce DC phynotypic and functional maturation as evidenced by higher expression of key surface molecules such as MHC II, CD80 and CD86. The functional tests proved the downregulation of ACP inside the DCs, occurred when phagocytosis of DCs decreased, with simultaneously antigen presentation increased toward maturation. Finally, PTD also stimulated production of more cytokine IL-12 and less TNF-α. Therefore it is concluded that PTD can markedly exert positive induction to murine DCs. |
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Authors:
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Xiaofang Hu; Wei Zhang; Lu Wang; Nan Wan; Bing Wang; Weiwei Li; Hui Hua; Xu Hu; Fengping Shan |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-01 |
Journal Detail:
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Title: Human vaccines & immunotherapeutics Volume: 8 ISSN: 2164-554X ISO Abbreviation: Hum Vaccin Immunother Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-8-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101572652 Medline TA: Hum Vaccin Immunother Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Experimental Laboratory; General Hospital of Shenyang Military Command; Shenyang, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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