| A derivative of myelin-associated glycoprotein in cerebrospinal fluid of normal subjects and patients with neurological disease. | |
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MedLine Citation:
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PMID: 2416268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A procedure for quantitating the myelin-associated glycoprotein (MAG) in cerebrospinal fluid (CSF) was developed. The procedure involved immunoprecipitation with rabbit polyclonal anti-MAG antiserum followed by immune staining of electroblots with the monoclonal antibody HNK-1. This method could detect as little as 2 ng of MAG per ml of CSF and could accurately measure levels of 5 ng/ml or greater. No intact MAG was detected in any of the CSF samples examined, but all samples contained the 90K dalton proteolytic derivative of MAG called dMAG. Samples from normal volunteers and patients with demyelinating diseases contained levels of dMAG ranging from 2 to 13 ng/ml. There was no correlation of dMAG levels with levels of myelin basic protein or with active demyelinating disease. The relatively high levels of dMAG, even in control CSF samples, probably resulted from normal physiological turnover of MAG and could possibly obscure any additional increments of MAG or dMAG release that might occur as a result of demyelination. |
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Authors:
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K Yanagisawa; R H Quarles; D Johnson; R O Brady; J N Whitaker |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Annals of neurology Volume: 18 ISSN: 0364-5134 ISO Abbreviation: Ann. Neurol. Publication Date: 1985 Oct |
Date Detail:
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Created Date: 1986-01-21 Completed Date: 1986-01-21 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7707449 Medline TA: Ann Neurol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 464-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Demyelinating Diseases
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cerebrospinal fluid Humans Immunologic Techniques Myelin Proteins / cerebrospinal fluid Myelin-Associated Glycoprotein Nervous System Diseases / cerebrospinal fluid* Radioimmunoassay Reference Values |
| Chemical | |
Reg. No./Substance:
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0/Myelin Proteins; 0/Myelin-Associated Glycoprotein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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