| The depressive effects of 5,8,11-eicosatrienoic Acid (20:3n-9) on osteoblasts. | |
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MedLine Citation:
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PMID: 18941818 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In cases of essential fatty acid deficiency, 5,8,11-eicosatrienoic acid (Mead acid, 20:3n-9) is synthesized from oleic acid as a 20-carbon analog of arachidonic acid. It was reported that 20:3n-9 levels were markedly higher in human fetal cartilage than in the muscle, liver and spleen. We, therefore, hypothesized that 20:3n-9 decreased osteoblastic activity. Goldfish scales were incubated either with 20:3n-9 or with oleic acid at 15 degrees C for 6 and 18 h. Both osteoblastic and osteoclastic activities in the scale were assessed by measuring alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase, respectively. MC3T3-E1 cells (an osteoblast cell line derived from the mouse) were incubated with 20:3n-9 or oleic acid at 37 degrees C for 6 and 18 h. ALP activity in cell lysate was measured. In the case of experiments with scales, 20:3n-9 (1-100 muM) significantly suppressed osteoblastic activity after 6 and 18 h of incubation, whereas oleic acid did not change this activity. Osteoclastic activity was not affected either by 20:3n-9 or by oleic acid. In the case with the cell line, osteoblastic activity was again significantly decreased with 20:3n-9 (10-30 muM) after 6-h incubation but not after 18 h incubation. The presence of 20:3n-9 in fetal cartilage may be important for the prevention of calcification in the cartilage. 20:3n-9 could be applied to some clinical situations where bone formation should be inhibited. |
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Authors:
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Tomohito Hamazaki; Nobuo Suzuki; Retno Widyowati; Tatsuro Miyahara; Shigetoshi Kadota; Hiroshi Ochiai; Kei Hamazaki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-22 |
Journal Detail:
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Title: Lipids Volume: 44 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-02-02 Completed Date: 2009-04-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: United States |
Other Details:
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Languages: eng Pagination: 97-102 Citation Subset: IM |
Affiliation:
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Department of Clinical Sciences, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, Toyama 9300194, Japan. hamazaki@inm.u-toyama.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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8,11,14-Eicosatrienoic Acid
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analogs & derivatives*,
pharmacology Acid Phosphatase / metabolism Alkaline Phosphatase / metabolism Animals Cell Line Depression, Chemical Female Goldfish Isoenzymes / metabolism Mice Oleic Acid / pharmacology Osteoblasts / drug effects*, physiology Osteoclasts / drug effects, physiology |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 112-80-1/Oleic Acid; 2751-14-6/mead acid; 7324-41-6/8,11,14-Eicosatrienoic Acid; EC 3.1.3.-/tartrate-resistant acid phosphatase; EC 3.1.3.1/Alkaline Phosphatase; EC 3.1.3.2/Acid Phosphatase |
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