| Icm/dot-dependent upregulation of phagocytosis by Legionella pneumophila. | |
| | |
MedLine Citation:
|
PMID: 11722729 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Legionella pneumophila is the causative agent of Legionnaires' disease, a severe pneumonia. Dependent on the icm/dot loci, L. pneumophila survives and replicates in macrophages and amoebae within a specialized phagosome that does not fuse with lysosomes. Here, we report that phagocytosis of wild-type L. pneumophila is more efficient than uptake of icm/dot mutants. Compared with the wild-type strain JR32, about 10 times fewer icm/dot mutant bacteria were recovered from HL-60 macrophages in a gentamicin protection assay. The defect in phagocytosis of the mutants could be complemented by supplying the corresponding genes on a plasmid. Using fluorescence microscopy and green fluorescent protein (GFP)-expressing strains, 10-20 times fewer icm/dot mutant bacteria were found to be internalized by HL-60 cells and human monocyte-derived macrophages (HMMPhi). Compared with icm/dot mutants, wild-type L. pneumophila infected two to three times more macrophages and yielded a population of highly infected host cells (15-70 bacteria per macrophage) that was not observed with icm/dot mutant strains. Wild-type and icmT mutant bacteria were found to adhere similarly and compete for binding to HMMPhi. In addition, wild-type L. pneumophila was also phagocytosed more efficiently by Acanthamoeba castellanii, indicating that the process is independent of adherence receptor(s). Wild-type L. pneumophila enhanced phagocytosis of an icmT mutant strain in a synchronous co-infection, suggesting that increased phagocytosis results from (a) secreted effector(s) acting in trans. |
| | |
Authors:
|
H Hilbi; G Segal; H A Shuman |
Related Documents
:
|
3582659 - Conjugation-dependent recovery of the na+ pump in a mutant of vibrio alginolyticus lack... 16535429 - Complete sucrose metabolism requires fructose phosphotransferase activity in corynebact... 16899219 - Regulatory roles of spnt, a novel gene located within transposon tntir. 21216029 - Meticillin-resistant staphylococcus aureus infection - the infectious stigma of our time? 18474589 - Increased resistance of complex i mutants to phytosphingosine-induced programmed cell d... 11500479 - Isolation and characterization of a vibrio vulnificus mutant deficient in both extracel... 23125319 - Salinisphaera japonica sp. nov., a novel moderately halophilic bacterium isolated from ... 9383279 - Simulation of the distribution of parental strains' genomes in rc strains of mice. 56269 - A device used for study of postural reactions in the quadruped. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Molecular microbiology Volume: 42 ISSN: 0950-382X ISO Abbreviation: Mol. Microbiol. Publication Date: 2001 Nov |
Date Detail:
|
Created Date: 2001-11-27 Completed Date: 2002-02-11 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: England |
Other Details:
|
Languages: eng Pagination: 603-17 Citation Subset: IM |
Affiliation:
|
Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acanthamoeba
/
microbiology Animals Anti-Bacterial Agents / pharmacology Bacterial Adhesion Bacterial Proteins / genetics, metabolism Carrier Proteins / genetics, metabolism* Cell Line Gentamicins / pharmacology Humans Legionella pneumophila / genetics, metabolism, physiology* Macrophages / microbiology* Membrane Proteins / genetics, metabolism* Mice Microscopy, Fluorescence Mutation Phagocytosis / physiology* Up-Regulation |
| Grant Support | |
ID/Acronym/Agency:
|
AI 23549/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/Carrier Proteins; 0/Gentamicins; 0/Membrane Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Discovery and distribution of super-integrons among pseudomonads.
Next Document: Protected environments allow parallel evolution of a bacterial pathogen in a patient subjected to lo...