Document Detail

The dependence of hepatic function upon sufficient oxygen supply during prolonged isolated rat liver perfusion.
MedLine Citation:
PMID:  9845297     Owner:  NLM     Status:  MEDLINE    
It is unclear how the omission of a perfusate oxygen carrier may influence data derived from prolonged in vitro liver perfusions. The effects of reduced perfusate oxygen content upon liver function tests conducted in livers from male Sprague-Dawley rats perfused for 6 h, were studied using diluted rat blood (PCV = 10%) (group 1) and compared to lines perfused with Krebs' buffer (group 2). Perfusate and bile samples were withdrawn at the start and at hourly intervals following the commencement of perfusion for biochemical analysis of aspartate-serine transaminase, alkaline phosphatase, bilirubin, urea and potassium concentrations, and also for perfusate gas measurements for calculations of hepatic oxygen uptake. There were no significant differences between the initial perfusion pressures of 11.4+/-1.6 and 15.7+/-2.4 mm Hg at the start of perfusion (group 1 versus group 2, respectively). Maintenance of comparable perfusion pressures at the start of perfusion, however, resulted in a significant difference in the fixed flow rate between the two groups of 1.9+/-0.1 and 2.6+/-0.2* ml/min/g liver and resulted in significantly higher perfusion pressures in group 2 than in group 1 after 6 h of perfusion (18.2+/-2.0 versus 8.7+/-1.7 mmHg, respectively). Bile volume production was significantly greater in group 1 than in group 2, at 784+/-84 vs 458+/-75 microl/h** respectively. Hepatic oxygen uptake (HOU) was significantly greater in group 1 than in group 2, and maximal at 0.93+/-0.13 vs 0.32+/-0.08** micromol/min/g, respectively. Bile concentrations of bilirubin and potassium were significantly greater in group 1 than in group 2, 23.0+/-0.6 vs 4.9+/-0.4 micromol/l*** and 4.7+/-0.5 vs 1.1+/-0.2 mmol/l**, respectively. Perfusate concentrations of urea, bilirubin, and glucose were significantly higher in group 1 than group 2, 5.7+/-0.5 versus 1.4+/-0.2 micromol/l**; 2.4+/-0.5 versus 0.7+/-0.1 micromol/l** and 22.1+/-1.4 versus 17.8+/-0.9 micromol/l***. It is concluded that in vitro perfusion of the rat liver with Krebs'-Henseleit buffer (KHB) via the portal vein alone may be insufficient to maintain optimum liver function as assessed by the tests used. Inclusion of a perfusate oxygen carrier is optimal if livers are to be perfused at physiological pressures. *p < 0.05; **p < 0.01; ***p < 0.001; group 1 versus group 2, Student's unpaired t test.
B Alexander; M Aslam; I S Benjamin
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmacological and toxicological methods     Volume:  39     ISSN:  1056-8719     ISO Abbreviation:  J Pharmacol Toxicol Methods     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1999-02-24     Completed Date:  1999-02-24     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  9206091     Medline TA:  J Pharmacol Toxicol Methods     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  185-92     Citation Subset:  IM    
Department of Surgery, King's College School of Medicine and Dentistry, Rayne Institute, London, Great Britain.
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MeSH Terms
Alkaline Phosphatase / metabolism
Bile / drug effects,  metabolism
Liver / chemistry,  metabolism,  physiology*
Liver Function Tests*
Oxygen / administration & dosage
Oxygen Consumption / physiology*
Potassium / pharmacology
Rats, Sprague-Dawley
Grant Support
//Wellcome Trust
Reg. No./Substance:
7440-09-7/Potassium; 7782-44-7/Oxygen; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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