| The dependence of enhanced lysosomal activity on the cellular aging of bovine aortic endothelial cells. | |
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MedLine Citation:
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PMID: 20174960 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lysosomes are a cell organelle type processing antimicrobial activity. Here, we investigate the lysosomal activity in a primary cell line, bovine aortic endothelial cells (BAECs), during cellular aging, based on the antimicrobial activity of lysosomes isolated from BAECs at cell passages 4, 6, 8, and 10. Cytochemical analysis of lysosomes with LysoTracker reagent revealed the number of lysosome-like organelles surrounding the nucleus initially increased drastically in the BAECs and continued increasing gradually until passage 10. The lysosomes isolated from each successive passage of BAECs exhibited increased antimicrobial activity against Escherichia coli, and, in addition, an age-dependent increase in lysosome intensity coincided with increased lysosomal antimicrobial activity. |
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Authors:
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Jihee Yoon; Ki-Ju Kim; Young-Woo Choi; Hwa Sung Shin; Yang-Hoon Kim; Jiho Min |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-20 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 340 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-15 Completed Date: 2010-09-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 175-8 Citation Subset: IM |
Affiliation:
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Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561-756, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amines Animals Aorta / cytology, enzymology Cattle Cell Aging* Cell Proliferation Cells, Cultured Colony Count, Microbial Endothelial Cells / enzymology* Escherichia coli / growth & development, metabolism Fluorescent Dyes Lysosomes / enzymology* Microscopy, Confocal Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Amines; 0/Fluorescent Dyes; 0/Red DND-99 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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