Document Detail

On-demand killing of adherent cells on photo-acid-generating culture substrates.
MedLine Citation:
PMID:  22833352     Owner:  NLM     Status:  Publisher    
As a powerful tool of cell screening and cell purification, we developed a novel method to kill adherent cells as cultured on a substrate by micro-projection of incoherent visible light. To kill the cells by the mild light irradiated by electrically controllable micro-projection systems currently available, we introduced the assist of the photo-responsive culture substrates functionalized with a photo-acid-generating (PAG) polymer. In clear contrast to the existing laser-based methods requiring point scanning, areal micro-prjection of blue light with the wavelength 436 nm killed many CHO-K1 cells at a time in the irradiated area on the substrate. The effect of the photo-generated acid was so confined that selective killing of targeted cells was achieved without critical damage to the neighboring cells. Further, we demonstrated the photo-selective killing of the adherent cells after preliminarily patterning through the photo-induced removal of cell adhesion-inhibiting polymer. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc.
Kimio Sumaru; Kyoko Kikuchi; Toshiyuki Takagi; Manae Yamaguchi; Taku Satoh; Kana Morishita; Toshiyuki Kanamori
Publication Detail:
Type:  LETTER     Date:  2012-7-25
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  -     ISSN:  1097-0290     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
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