Document Detail

A delta9 desaturase from Bombus lucorum males: investigation of the biosynthetic pathway of marking pheromones.
MedLine Citation:
PMID:  18785199     Owner:  NLM     Status:  MEDLINE    
The knowledge of the molecular basis of communication in bumblebee communities is limited. None of the enzymes that participate in pheromone production have been characterized. Here, we cloned the gene encoding the Delta(9) desaturase from cDNA prepared from the total RNA of the pheromone gland and fat bodies of Bombus lucorum male. Functional expression of BlucNPVE desaturase in Saccharomyces cerevisiae and GC-MS analyses revealed its preference for C(18) saturated fatty acids. This suggests that Delta(9) desaturase is involved in the desaturation of metabolic fatty acids stored in triacylglyceroles (TAGs), because oleic acid is the most abundant fatty acid bound in TAG in B. lucorum and it is present in low concentration in the pheromone blend. The incubation of pheromone precursors with a dissected labial gland as well as direct injection of labelled pheromone substrates into B. lucorum males revealed that esterification of pheromone products occurs in the labial gland. These results support both the biosynthesis of pheromones from common lipids and the de novo synthesis of unsaturated pheromones in the labial gland.
Petra Matousková; Anna Luxová; Jana Matousková; Pavel Jiros; Ales Svatos; Irena Valterová; Iva Pichová
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chembiochem : a European journal of chemical biology     Volume:  9     ISSN:  1439-7633     ISO Abbreviation:  Chembiochem     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-13     Completed Date:  2008-12-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100937360     Medline TA:  Chembiochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2534-41     Citation Subset:  IM    
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic.
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MeSH Terms
Amino Acid Sequence
Bees / chemistry,  enzymology*
Cloning, Molecular
Esters / metabolism
Molecular Sequence Data
Sequence Alignment
Sequence Homology, Amino Acid
Sex Attractants / biosynthesis*,  chemistry
Stearoyl-CoA Desaturase / chemistry,  genetics,  metabolism*
Reg. No./Substance:
0/Esters; 0/Sex Attractants; EC Desaturase

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