Document Detail

The delta 5-3 beta-hydroxy steroid acyl transferase activities in tissues of the male rat and sheep.
MedLine Citation:
PMID:  9178429     Owner:  NLM     Status:  MEDLINE    
Pregnenolone and dehydroepiandrosterone and their sulphate and fatty acid ester derivatives are concentrated in the mammalian brain, as compared to the peripheral organs. Although biological functions have been postulated for the free steroid and sulphate conjugates, the role of steroid fatty acid esters in the central nervous system (CNS) has yet to be established. A comparison of the esterifying capacities of brain and peripheral organs of the male rat and sheep is made here. The male rat brain was found to possess a higher esterifying capacity than all other tissues examined, with 5.32 +/- 2.46 pmol dehydroepiandrosterone fatty acid esters synthesized/mg protein/h and 1.89 +/- 0.42 pmol pregnenolone fatty acid esters synthesized/mg protein/h. Sheep adrenal and sheep liver homogenates were found to have higher esterifying capacities than sheep brain. Within the brain of both species. delta 5-3 beta-hydroxy steroid acyl transferase enzyme(s) were concentrated in the microsomal fraction. The addition of a lecithin:cholesterol acyl transferase inhibitor to incubation assays resulted in a loss in enzyme activity. This effect was more significant in sheep brain microsomes than rat brain microsomes. It is likely that lecithin:cholesterol acyl transferase is more active in the sheep brain than the rat brain. Results from this study indicate that the steroid acyl transferase enzymes that are active in the mammalian brain are substrate specific.
A J Smith; T G Watson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Steroids     Volume:  62     ISSN:  0039-128X     ISO Abbreviation:  Steroids     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-08-11     Completed Date:  1997-08-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404536     Medline TA:  Steroids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  422-6     Citation Subset:  IM    
School of Biological and Chemical Sciences, Deakin University, Geelong, Victoria, Australia.
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MeSH Terms
Acyltransferases / antagonists & inhibitors,  metabolism*
Brain / enzymology
Dehydroepiandrosterone / metabolism
Dithionitrobenzoic Acid / pharmacology
Hydroxysteroids / metabolism*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Hydroxysteroids; 53-43-0/Dehydroepiandrosterone; 69-78-3/Dithionitrobenzoic Acid; EC 2.3.-/Acyltransferases

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