Document Detail


The delivery of foreign genes into fertilized fish eggs using high-velocity microprojectiles.
MedLine Citation:
PMID:  1652457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fertilized eggs of loach (Misgurnus fossilis), rainbow trout (Salmo gairdneri) and zebrafish (Brachydanio rerio) were bombarded with high-velocity tungsten microprojectiles covered with plasmid DNA containing sequences of beta-galactosidase and neomycin phosphotransferase genes. About 70% of the eggs survived the bombardment. The activity of both transferred genes was revealed in the fish developed from the bombarded eggs. Neomycin phosphotransferase gene sequences were detected by means of PCR amplification and Southern hybridization in the total DNA of zebrafish that survived after G418 treatment.
Authors:
A V Zelenin; A A Alimov; V A Barmintzev; A O Beniumov; I A Zelenina; A M Krasnov; V A Kolesnikov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  287     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1991-09-27     Completed Date:  1991-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  118-20     Citation Subset:  IM    
Affiliation:
Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cypriniformes / embryology*
DNA / analysis
Gene Expression
Kanamycin Kinase
Larva / analysis
Microspheres
Phosphotransferases / genetics
Plasmids
Polymerase Chain Reaction
Transfection*
Trout / embryology*
Tungsten
Zebrafish / embryology*
Zygote*
beta-Galactosidase / genetics
Chemical
Reg. No./Substance:
7440-33-7/Tungsten; 9007-49-2/DNA; EC 2.7.-/Phosphotransferases; EC 2.7.1.95/Kanamycin Kinase; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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