| The delivery of foreign genes into fertilized fish eggs using high-velocity microprojectiles. | |
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MedLine Citation:
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PMID: 1652457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fertilized eggs of loach (Misgurnus fossilis), rainbow trout (Salmo gairdneri) and zebrafish (Brachydanio rerio) were bombarded with high-velocity tungsten microprojectiles covered with plasmid DNA containing sequences of beta-galactosidase and neomycin phosphotransferase genes. About 70% of the eggs survived the bombardment. The activity of both transferred genes was revealed in the fish developed from the bombarded eggs. Neomycin phosphotransferase gene sequences were detected by means of PCR amplification and Southern hybridization in the total DNA of zebrafish that survived after G418 treatment. |
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Authors:
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A V Zelenin; A A Alimov; V A Barmintzev; A O Beniumov; I A Zelenina; A M Krasnov; V A Kolesnikov |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: FEBS letters Volume: 287 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1991 Aug |
Date Detail:
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Created Date: 1991-09-27 Completed Date: 1991-09-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 118-20 Citation Subset: IM |
Affiliation:
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Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cypriniformes / embryology* DNA / analysis Gene Expression Kanamycin Kinase Larva / analysis Microspheres Phosphotransferases / genetics Plasmids Polymerase Chain Reaction Transfection* Trout / embryology* Tungsten Zebrafish / embryology* Zygote* beta-Galactosidase / genetics |
| Chemical | |
Reg. No./Substance:
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7440-33-7/Tungsten; 9007-49-2/DNA; EC 2.7.-/Phosphotransferases; EC 2.7.1.95/Kanamycin Kinase; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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