Document Detail


A deletion panel of the long arm of the X chromosome: subregional localization of 22 DNA probes.
MedLine Citation:
PMID:  1972695     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two males and two females with different but overlapping deletions on the proximal long arm of the X chromosomes have been investigated. Their karyotypes, which have been well characterized by high resolution banding techniques, are 46,Y,del(X)(pter----q21.1::q21.33----qter); 46,Y,del(X)(pter----q21.2::q21.31----qter); 46,X,del(X)(pter----q21.31::q24.3----qter) and 46,X,del(X)(pter----q21.1:). A deletion panel, which makes it possible to subdivide the long arm of the X chromosome into seven subregions, has been established using the genomic DNA from the four families, and applied to the fine subregional localization of the loci for 22 DNA probes. Based on the results obtained, the possible location of the loci in question has been narrowed down considerably, in some cases to an area of only 5% of the previously assigned region; hybridization to Southern blots of a panel with well-characterized chromosome deletions is thus a powerful means of localizing DNA probes, especially with respect to the X probes.
Authors:
H M Yang; T Lund; E Niebuhr; S Nørby; M Schwartz; L Shen
Related Documents :
20503325 - Unbalanced der(5)t(5;20) translocation associated with megalencephaly, perisylvian poly...
3706405 - Distal deletion of the long arm of chromosome 6: a specific phenotype?
16896345 - Ulnar-mammary syndrome with dysmorphic facies and mental retardation caused by a novel ...
20236125 - Identification and molecular characterization of two novel chromosomal deletions associ...
9702495 - Detection of numerical chromosome 17 abnormalities in fine-needle aspirates of breast c...
3729925 - Fourteen genetically variant proteins of mouse brain: discovery of two new variants and...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human genetics     Volume:  85     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-08-01     Completed Date:  1990-08-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  25-30     Citation Subset:  IM    
Affiliation:
Institute of Medical Genetics, University of Copenhagen, Denmark.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Blotting, Southern
Cells, Cultured
Chromosome Deletion*
Chromosome Mapping
DNA Probes / genetics*
Female
Humans
Male
Pedigree
Polymorphism, Restriction Fragment Length
X Chromosome*
Chemical
Reg. No./Substance:
0/DNA Probes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Constitutional deletions predisposing to retinoblastoma.
Next Document:  Assignment of the apolipoprotein A-I gene to 11q23 based on RFLP in a case with a partial deletion o...