Document Detail

A deficiency of gastric interstitial cells of Cajal accompanied by decreased expression of neuronal nitric oxide synthase and substance P in patients with type 2 diabetes mellitus.
MedLine Citation:
PMID:  17160518     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Gastrointestinal motility is impaired in patients with diabetes mellitus (DM). Interstitial cells of Cajal (ICC) in the gastrointestinal tract play a central role in gastrointestinal motility. The present study examined whether ICC density, or expression of neuronal nitric oxide synthase (nNOS)- and substance P (SP)-containing nerves in the gastric antrum, were altered in patients with type 2 DM. METHODS: Paraffin-embedded gastric specimens from 51 controls and 36 male DM patients with gastric cancer were used for immunohistochemistry. Serial sections were stained with Kit and mast cell tryptase-specific antibodies. Fresh-frozen gastric specimens from patients with gastric cancer were used for immunofluorescence. The specimens were stained with antibodies to Kit, nNOS, and SP, and levels of expression of these three markers were compared between controls and DM patients. RESULTS: ICC density in the inner circular muscle layer, but not in the myenteric plexus, was lower in patients with severe DM than in controls in paraffin-embedded specimens. In addition, decreased expression of nNOS and SP accompanied by reduced ICC density was observed in frozen specimens from patients with DM. CONCLUSIONS: These results suggest that lower gastric ICC, nNOS, and SP densities in patients with DM may be associated with the pathogenesis of diabetic gastroparesis.
Hirohiko Iwasaki; Masayoshi Kajimura; Satoshi Osawa; Shigeru Kanaoka; Takahisa Furuta; Mutsuhiro Ikuma; Akira Hishida
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-08
Journal Detail:
Title:  Journal of gastroenterology     Volume:  41     ISSN:  0944-1174     ISO Abbreviation:  J. Gastroenterol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-12-12     Completed Date:  2007-02-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430794     Medline TA:  J Gastroenterol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1076-87     Citation Subset:  IM    
First Department of Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan.
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MeSH Terms
Biological Markers
Cell Count
Diabetes Mellitus, Type 2 / metabolism*,  pathology,  physiopathology
Gastric Emptying / physiology
Gastric Mucosa / innervation*,  metabolism
Microscopy, Confocal
Myenteric Plexus / metabolism,  pathology*
Neurons / metabolism*,  pathology
Nitric Oxide Synthase Type I / biosynthesis*
Retrospective Studies
Substance P / biosynthesis*
Reg. No./Substance:
0/Biological Markers; 33507-63-0/Substance P; EC Oxide Synthase Type I
Comment In:
J Gastroenterol. 2006 Nov;41(11):1128-30   [PMID:  17160526 ]

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