Document Detail


The decarboxylation of the weak-acid preservative, sorbic acid, is encoded by linked genes in Aspergillus spp.
MedLine Citation:
PMID:  20452450     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability to resist anti-microbial compounds is of key evolutionary benefit to microorganisms. Aspergillus niger has previously been shown to require the activity of a phenylacrylic acid decarboxylase (encoded by padA1) for the decarboxylation of the weak-acid preservative sorbic acid (2,4-hexadienoic acid) to 1,3-pentadiene. It is now shown that this decarboxylation process also requires the activity of a putative 4-hydroxybenzoic acid (3-octaprenyl-4-hydroxybenzoic acid) decarboxylase, encoded by a gene termed ohbA1, and a putative transcription factor, sorbic acid decarboxylase regulator, encoded by sdrA. The padA1,ohbA1 and sdrA genes are in close proximity to each other on chromosome 6 in the A. niger genome and further bioinformatic analysis revealed conserved synteny at this locus in several Aspergillus species and other ascomycete fungi indicating clustering of metabolic function. This cluster is absent from the genomes of A. fumigatus and A. clavatus and, as a consequence, neither species is capable of decarboxylating sorbic acid.
Authors:
Andrew Plumridge; Petter Melin; Malcolm Stratford; Michaela Novodvorska; Lee Shunburne; Paul S Dyer; Johannes A Roubos; Hildegard Menke; Jacques Stark; Hein Stam; David B Archer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-07
Journal Detail:
Title:  Fungal genetics and biology : FG & B     Volume:  47     ISSN:  1096-0937     ISO Abbreviation:  Fungal Genet. Biol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-08     Completed Date:  2010-10-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607601     Medline TA:  Fungal Genet Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  683-92     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Affiliation:
School of Biology, University Park, University of Nottingham, Nottingham NG7 2RD, UK.
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MeSH Terms
Descriptor/Qualifier:
Alkadienes / metabolism
Amino Acid Sequence
Antifungal Agents / metabolism*
Aspergillus niger / metabolism*
Carboxy-Lyases / genetics
Decarboxylation
Fungal Proteins / genetics
Gene Order
Genes, Fungal*
Metabolic Networks and Pathways / genetics*
Microbial Sensitivity Tests
Molecular Sequence Data
Multigene Family
Parabens / metabolism
Pentanes / metabolism
Sequence Alignment
Sorbic Acid / metabolism*
Synteny
Transcription Factors / genetics
Grant Support
ID/Acronym/Agency:
//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Alkadienes; 0/Antifungal Agents; 0/Fungal Proteins; 0/Parabens; 0/Pentanes; 0/Transcription Factors; 110-44-1/Sorbic Acid; 504-60-9/1,3-pentadiene; 99-96-7/4-hydroxybenzoic acid; EC 4.1.1.-/Carboxy-Lyases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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