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d-pinitol inhibits RANKL-induced osteoclastogenesis.
MedLine Citation:
PMID:  22269833     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Numerous studies have indicated that inflammatory cytokines play a major role in osteoclastogenesis, leading to the bone resorption that is frequently associated with osteoporosis. d-pinitol, a 3-methoxy analogue of d-chiroinositol, was identified as an active principle in soy foods and legumes. Here we found that d-pinitol markedly inhibited the receptor activator of nuclear factor kappa B ligand (RANKL)-induced osteoclastic differentiation from bone marrow stromal cells and RAW264.7 macrophage cells. In addition, d-pinitol also reduced RANKL-induced p38 and JNK phosphorylation. Furthermore, RANKL-mediated increase of IKK, IκBα, and p65 phosphorylation and NF-κB-luciferase activity was inhibited by d-pinitol. However, d-pinitol did not affect the proliferation and differentiation of osteoblasts. In addition, d-pinitol also prevented the bone loss induced by ovariectomy in vivo. Our data suggest that d-pinitol inhibits osteoclastogenesis from bone marrow stromal cells and macrophage cells via attenuated RANKL-induced p38, JNK, and NF-κB activation, which in turn protect bone loss from ovariectomy.
Authors:
Shan-Chi Liu; Show-Mei Chuang; Chih-Hsin Tang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-20
Journal Detail:
Title:  International immunopharmacology     Volume:  -     ISSN:  1878-1705     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100965259     Medline TA:  Int Immunopharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan.
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