| D-Ornithine coopts pyrrolysine biosynthesis to make and insert pyrroline-carboxy-lysine. | |
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MedLine Citation:
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PMID: 21525873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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D-ornithine has previously been suggested to enhance the expression of pyrrolysine-containing proteins. We unexpectedly discovered that uptake of D-ornithine results in the insertion of a new amino acid, pyrroline-carboxy-lysine (Pcl) instead of the anticipated pyrrolysine (Pyl). Our feeding and biochemical studies point to specific roles of the poorly understood Pyl biosynthetic enzymes PylC and PylD in converting L-lysine and D-ornithine to Pcl and confirm intermediates in the biosynthesis of Pyl. |
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Authors:
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Susan E Cellitti; Weijia Ou; Hsien-Po Chiu; Jan Grünewald; David H Jones; Xueshi Hao; Qian Fan; Lisa L Quinn; Kenneth Ng; Andrew T Anfora; Scott A Lesley; Tetsuo Uno; Ansgar Brock; Bernhard H Geierstanger |
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Publication Detail:
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Type: Journal Article Date: 2011-04-27 |
Journal Detail:
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Title: Nature chemical biology Volume: 7 ISSN: 1552-4469 ISO Abbreviation: Nat. Chem. Biol. Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-07-19 Completed Date: 2011-09-29 Revised Date: 2011-11-28 |
Medline Journal Info:
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Nlm Unique ID: 101231976 Medline TA: Nat Chem Biol Country: United States |
Other Details:
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Languages: eng Pagination: 528-30 Citation Subset: IM |
Affiliation:
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Genomics Institute of the Novartis Research Foundation, San Diego, California, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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PubChem-Substance/118111069; 118111070; 118111071; 118111072; 118111073; 118111074; 118111075; 118111076; 118111077; 118111078; 118111079; 118111080; 118111081; 118111082; 118111083; 118111084; 118111085; 118111086; 118111087; 118111088; 118111089; 118111090; 118111091 |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Escherichia coli Gene Expression Regulation / physiology HEK293 Cells Humans Lysine / analogs & derivatives*, biosynthesis, chemistry Methanosarcina / genetics, metabolism Molecular Structure Ornithine / chemistry, metabolism, pharmacology* Plasmids Promoter Regions, Genetic |
| Chemical | |
Reg. No./Substance:
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0/2-amino-6-(3,4-dihydro-2H-pyrrole-2-carboxamido)hexanoic acid; 0/pyrrolysine; 56-87-1/Lysine; 7006-33-9/Ornithine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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