| d-Lactic acid interferes with the effects of platelet activating factor on bovine neutrophils. | |
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MedLine Citation:
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PMID: 21816488 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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d-Lactic acidosis occurs in ruminants, such as cattle, with acute ruminal acidosis caused by ingestion of excessive amounts of highly fermentable carbohydrates. Affected animals show clinical signs similar to those of septic shock, as well as acute laminitis and liver abscesses. It has been proposed that the inflammatory response and susceptibility to infection could both be caused by the inhibition of phagocytic mechanisms. To determine the effects of d-lactic acid on bovine neutrophil functions, we pretreated cells with different concentrations of d-lactic acid and measured intracellular pH using 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) and calcium flux using FLUO-3 AM-loaded neutrophils. Reactive oxygen species (ROS) production was measured using a luminol chemiluminescence assay, and MMP-9/gelatinase-B granule release was measured by zymography. CD11b and CD62L/l-selectin expression, changes in cell shape, superoxide anion production, phagocytosis of Escherichia coli-Texas red bioparticles, and apoptosis were all measured using flow cytometry. Our results demonstrated that d-lactic acid reduced ROS production, CD11b upregulation and MMP-9 release in bovine neutrophils treated with 100nM platelet-activating factor (PAF). d-Lactic acid induced MMP-9 release and, at higher concentrations, upregulated CD11b expression, decrease l-selectin expression, and induces late apoptosis. We concluded that d-lactic acid can interfere with neutrophil functions induced by PAF, leading to reduced innate immune responses during bacterial infections. Moreover, the increase of MMP-9 release and CD11b expression induced by 10mM d-lactic acid could promote an nonspecific neutrophil-dependent inflammatory reaction in cattle with acute ruminal acidosis. |
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Authors:
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P Alarcón; I Conejeros; M D Carretta; C Concha; E Jara; N Tadich; M A Hidalgo; R A Burgos |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-23 |
Journal Detail:
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Title: Veterinary immunology and immunopathology Volume: - ISSN: 1873-2534 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-8-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8002006 Medline TA: Vet Immunol Immunopathol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Laboratory of Inflammation Pharmacology, Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Science, Universidad Austral de Chile, P.O. Box 567, Valdivia, Chile. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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