Document Detail


d-Amino acids in aged proteins: Analysis and biological relevance.
MedLine Citation:
PMID:  21723211     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Homochirality is essential for life. l-Amino acids are exclusively used as substrates for the polymerization and formation of peptides and proteins in living systems. However, d-amino acids, which are enantiomers of l-amino acids, were recently detected in various living organisms in the form of free d-amino acids and d-amino acid residues in peptides and proteins. In particular, d-aspartyl (Asp) residues have been detected in various proteins from diverse tissues of elderly individuals. Here, we describe three important aspects of our research: (i) a method for detecting d-β-Asp at specific sites in particular proteins, (ii) a likely spontaneous mechanism by which Asp residues in proteins invert and isomerize to the d-β-form with age under physiological conditions, (iii) a discussion of factors that favor such a reaction.
Authors:
Noriko Fujii; Yuichi Kaji; Norihiko Fujii
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-12
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  -     ISSN:  1873-376X     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-7-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Research Reactor Institute, Kyoto University, Kumatori, Sennan, Osaka 590-0494, Japan.
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