Document Detail


The cytotoxic and mutagenic effects of 5-bromodeoxyuridine-plus-black-light treatment in cultured mammalian cells.
MedLine Citation:
PMID:  7099189     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cytotoxic and mutagenic effects of the incorporation of 5-bromodeoxyuridine (BrdU) followed by exposure to black light were investigated with Chinese hamster ovary (CHO) cells in cell culture. Mutation induction at the hypoxanthine-guanine phosphoribosyl transferase (hgprt) locus was determined by selection for 6-thioguanine resistant (TGr) mutants (CHO/HGPRT system). BrdU alone has been shown to be mutagenic only at concentrations of 50 micro M or greater. This study was performed in an effort to determine whether BrdU is actually incorporated into the hgprt gene when lower, nonmutagenic concentrations are employed. Neither BrdU (1-20 micro M) nor exposure to black light alone was mutagenic, but the combined treatment did result in the induction of TGr mutants. The mutant frequency increased with increasing light exposure at constant BrdU and with increasing BrdU at constant light exposure. These results show that BrdU is incorporated into the hgprt gene, but that this does not result in mutation induction in the absence of light exposure. Such a BrdU-plus-light procedure might be applied to studies of DNA repair at this locus, since mutation induction requires both BrdU incorporation and subsequent exposure to black light.
Authors:
L A Lynn; J P O'Neill
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Mutation research     Volume:  94     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1982 May 
Date Detail:
Created Date:  1982-09-10     Completed Date:  1982-09-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  179-87     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bromodeoxyuridine / pharmacology*
Cell Survival
Cells, Cultured
Cricetinae
Cricetulus
Female
Fibroblasts / drug effects*,  radiation effects
Hypoxanthine Phosphoribosyltransferase / genetics
Mutagens / pharmacology*
Ovary
Photochemistry
Ultraviolet Rays
Chemical
Reg. No./Substance:
0/Mutagens; 59-14-3/Bromodeoxyuridine; EC 2.4.2.8/Hypoxanthine Phosphoribosyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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