Document Detail

The cytotoxic effects of human neutrophil peptide-1 (HNP1) and lactoferrin on oral squamous cell carcinoma (OSCC) in vitro.
MedLine Citation:
PMID:  16483834     Owner:  NLM     Status:  MEDLINE    
Alpha-defensin or human neutrophil peptide-1 (HNP1) is a neutrophil-derived antimicrobial peptide with cytotoxic effects towards cancer cells. Lactoferrin is also stored in human neutrophils and is a glycoprotein involved in mediating cytotoxicity towards tumour cells. This study investigated the sensitivity of normal oral keratinocyte and oral squamous cell carcinoma (OSCC) cells to HNP1 and lactoferrin in various combinations. A concentration of 100 microg/ml HNP1 induced the most significant cytotoxic effect on both normal and OSCC cells. Lactoferrin (12.5, 25 and 250 microg/ml) also significantly induced cell death in OSCC cells after 72 h. Of note, a combination of 10 microg/ml HNP1 and 50 microg/ml lactoferrin induced a differential effect, not observed with either concentration alone, which stimulated proliferation in normal cells, but induced cell death in OSCC cells throughout the study. These results indicate a potentially important co-operative role for HNP1 and lactoferrin in facilitating a selective cytotoxic effect on tumour cells.
Scott T W McKeown; Fionnuala T Lundy; John Nelson; Derek Lockhart; Chris R Irwin; C Gerard Cowan; John J Marley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-17
Journal Detail:
Title:  Oral oncology     Volume:  42     ISSN:  1368-8375     ISO Abbreviation:  Oral Oncol.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-31     Completed Date:  2007-06-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  England    
Other Details:
Languages:  eng     Pagination:  685-90     Citation Subset:  IM    
Oral Science Research Centre, School of Medicine and Dentistry, Queen's University Belfast, Royal Victoria Hospital, Grosvenor Road, Belfast BT12 6BP, Northern Ireland, United Kingdom.
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MeSH Terms
Carcinoma, Squamous Cell / enzymology,  pathology*
Cell Death / drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Drug Interactions
Drug Screening Assays, Antitumor / methods
Electrophoresis, Polyacrylamide Gel
Keratinocytes / drug effects,  enzymology
Lactoferrin / pharmacology*
Mouth Mucosa / cytology,  drug effects,  enzymology
Mouth Neoplasms / enzymology,  pathology*
Tumor Cells, Cultured
alpha-Defensins / pharmacology*
Reg. No./Substance:
0/Lactoferrin; 0/alpha-Defensins; 0/human neutrophil peptide 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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