| The cytotoxic effect of emetine and CGP-74514A studied with the Hollow Fiber Model and ArrayScan Assay in neuroendocrine tumors In Vitro. | |
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MedLine Citation:
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PMID: 22263790 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Emetine and CGP-74514A has previously shown antitumor activity in neuroendocrine tumor cell lines. The aim of this study was to investigate the cytotoxic activity of the drugs in a three-dimensional model and to study if the mechanism of the cytotoxic activity was induction of apoptosis. An in vitro hollow fiber model was used to study the cytotoxic effect and a multiparametric high-content screening assay was used for measurement of apoptosis. The human pancreatic carcinoid cell line, BON-1 and the human typical and atypical bronchial carcinoid cell lines NCI-H727 and NCI-H720 were tested. Emetine and CGP-74514A showed higher antitumor activity on NCI-H720 compared to NCI-H727 and 3 day cultures were more sensitive than the 14 day cultures. A time- and dose-dependent activation of caspase-3 and increase in nuclear fragmentation and condensation were observed for the drugs in NCI-H727 and BON-1 using a multiparametric apoptosis assay. These results were confirmed with nuclear morphological examinations on microscopic slides. Emetine and CGP-74514A showed antitumor activity and induced caspase-3 activation with modest changes in nuclear morphology, indicating induction of apoptosis. |
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Authors:
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Dhana E Larsson; Saadia B Hassan; Kjell Oberg; Dan Granberg |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-19 |
Journal Detail:
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Title: Anti-cancer agents in medicinal chemistry Volume: - ISSN: 1875-5992 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101265649 Medline TA: Anticancer Agents Med Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Medical Sciences, Section of Endocrine Oncology, University Hospital, S-751 85 Uppsala, Sweden. dhana.larsson@medsci.uu.se. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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