Document Detail


A cytosolic phospholipase in human neutrophils that hydrolyzes arachidonoyl-containing phosphatidylcholine.
MedLine Citation:
PMID:  3092867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In stimulated neutrophils the production of eicosinoids and the lipid mediator, platelet-activating factor, is thought to be initiated by the activation of a phospholipase A2 which cleaves arachidonic acid from choline-containing glycerophospholipids. Accordingly, studies were undertaken in human neutrophils to characterize phospholipase enzymes that can hydrolyze 1-acyl- and 1-alkyl-linked arachidonoyl-containing phosphatidylcholine (PC). Cellular homogenates were incubated with sonicated dispersions of the arachidonoyl-labeled phospholipid substrates and the hydrolysis of radiolabeled arachidonate was measured. The phospholipase activity was cytosolic, optimal at pH 8.0, and calcium dependent. The homogenization conditions used were important in determining the amount of recoverable enzymatic activity. Vigorous sonication and the presence of calcium during homogenization were strongly inhibitory, whereas the presence of EGTA, heparin and proteinase inhibitors during homogenization increased the activity. Competitive experiments with unlabeled substrates suggested that the phospholipase hydrolyzed arachidonic acid equally well from either 1-acyl- or 1-alkyl-linked PC. However, the phospholipase did show specificity for arachidonic acid, compared to oleic or linoleic acids, at the sn-2 position of 1-acyl-linked PC. When neutrophils were first stimulated with the ionophore A23187, the phospholipase activity against 1-O-hexadecyl-2-[3H]arachidonoylglycerophosphocholine (GPC) increased in a time-dependent fashion up to 3.5-fold over the unstimulated level. The activity against 1-palmitoyl-2-[3H]arachidonoyl-GPC also increased after ionophore stimulation but to a lesser extent. The results demonstrate the presence of a cytosolic, activatable phospholipase that may be involved in PC turnover, arachidonic acid release, and platelet-activating factor production in human neutrophils.
Authors:
F Alonso; P M Henson; C C Leslie
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  878     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1986 Sep 
Date Detail:
Created Date:  1986-10-30     Completed Date:  1986-10-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  273-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Arachidonic Acid
Arachidonic Acids / metabolism*
Calcium / pharmacology
Cytosol / enzymology*
Heparin / pharmacology
Humans
Hydrogen-Ion Concentration
Hydrolysis
Neutrophils / enzymology*
Phosphatidylcholines / metabolism*
Phospholipases / analysis*
Platelet Activating Factor / biosynthesis
Structure-Activity Relationship
Substrate Specificity
Grant Support
ID/Acronym/Agency:
GM 24834/GM/NIGMS NIH HHS; HL 32015/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Phosphatidylcholines; 0/Platelet Activating Factor; 506-32-1/Arachidonic Acid; 7440-70-2/Calcium; 9005-49-6/Heparin; EC 3.1.-/Phospholipases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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