Document Detail


A cytoplasmic dynein required for mitotic aster formation in vivo.
MedLine Citation:
PMID:  9701559     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An astral pulling force helps to elongate the mitotic spindle in the filamentous ascomycete, Nectria haematococca. Evidence is mounting that dynein is required for the formation of mitotic spindles and asters. Obviously, this would be an important mitotic function of dynein, since it would be a prerequisite for astral force to be applied to a spindle pole. Missing from the evidence for such a role of dynein in aster formation, however, has been a dynein mutant lacking mitotic asters. To determine whether or not cytoplasmic dynein is involved in mitotic aster formation in N. haematococca, a dynein-deficient mutant was made. Immunocytochemistry visualized few or no mitotic astral microtubules in the mutant cells, and studies of living cells confirmed the veracity of this result by revealing the absence of mitotic aster functions in vivo: intra-astral motility of membranous organelles was not apparent; the rate and extent of spindle elongation during anaphase B were reduced; and spindle pole body separation almost stopped when the anaphase B spindle in the mutant was cut by a laser microbeam, demonstrating unequivocally that no astral pulling force was present. These unique results not only provide a demonstration that cytoplasmic dynein is required for the formation of mitotic asters in N. haematococca; they also represent the first report of mitotic phenotypes in a dynein mutant of any filamentous fungus and the first cytoplasmic dynein mutant of any organism whose mitotic phenotypes demonstrate the requirement of cytoplasmic dynein for aster formation in vivo.
Authors:
S Inoue; O C Yoder; B G Turgeon; J R Aist
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cell science     Volume:  111 ( Pt 17)     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1999-05-25     Completed Date:  1999-05-25     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2607-14     Citation Subset:  IM    
Affiliation:
Department of Plant Pathology, Cornell University, Ithaca, New York 14853, USA.
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MeSH Terms
Descriptor/Qualifier:
Anaphase / radiation effects
Cytoplasm / enzymology*
Dyneins / genetics,  metabolism*,  physiology*
Hypocreales / enzymology,  genetics
Immunohistochemistry
Laser Therapy
Microscopy, Interference
Microscopy, Video
Mitosis / genetics,  physiology
Mitotic Spindle Apparatus / enzymology*,  physiology*
Mutagenesis, Site-Directed
Phenotype
Grant Support
ID/Acronym/Agency:
P41-01219//PHS HHS
Chemical
Reg. No./Substance:
EC 3.6.4.2/Dyneins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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