Document Detail

The cytologic identification and quantification of testicular cell subtypes. Reproducibility and relation to histologic findings in the diagnosis of male infertility.
MedLine Citation:
PMID:  3421018     Owner:  NLM     Status:  MEDLINE    
In testicular imprint smears from 100 infertile men (both testicles), stained using the Pappenheim and Papanicolaou methods, the cell forms (light and dark spermatogonia, primary and secondary spermatocytes, spermatids, spermatozoa and Sertoli cells) were identified and quantified by the counting of 500 consecutive cells. Identification of the cell types, which are described and illustrated, was consistent and reproducible; the advantages of the different stainings in their analysis is documented. The cell counts were tested for reproducibility and compared to the histologic diagnoses and sperm counts. Statistical analysis showed the highest reproducibility for cells frequently encountered in smears (0.99 for Sertoli cells and 0.98 for spermatozoa) and the lowest but still satisfactory reproducibility for rare or arbitrarily defined cell forms (0.71 for dark spermatogonia and 0.76 for secondary spermatocytes). The high reproducibility of the smear quantification permits the introduction of a number of indices, defining clinically useful relations between cell types that are indicative of various types of infertility. The data obtained by cytologic quantification showed reasonably good correlation with the histologic diagnoses of desquamation and focal fibrosis and excellent correlation with Sertoli cells only, arrested spermatogenesis and complete fibrosis. The cytologic quantification of testicular smears adds considerable information to the diagnosis of impaired fertility and should be instituted in properly equipped centers.
Z Papić; G Katona; Z Skrabalo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta cytologica     Volume:  32     ISSN:  0001-5547     ISO Abbreviation:  Acta Cytol.     Publication Date:    1988 Sep-Oct
Date Detail:
Created Date:  1988-10-26     Completed Date:  1988-10-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370307     Medline TA:  Acta Cytol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  697-706     Citation Subset:  IM    
Department of Morphology, Vuk Vhrovac Institute for Diabetes, Endocrinology and Metabolic Diseases, Zagreb University School of Medicine, Yugoslavia.
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MeSH Terms
Cell Count
Infertility, Male / pathology*
Leydig Cells / pathology
Mitotic Index
Sertoli Cells / pathology
Sperm Count
Spermatids / pathology
Spermatocytes / pathology
Spermatogonia / pathology
Spermatozoa / classification*,  pathology
Testis / pathology*

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