Document Detail


The cytochrome c peroxidase.cytochrome c electron transfer complex. Experimental support of a hypothetical model.
MedLine Citation:
PMID:  6281255     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A hypothetical model of the cytochrome c peroxidase.cytochrome c complex (Poulos, T. L., and Kraut, J. (1980) J. Biol. Chem. 255, 10322-10330) predicts charge interactions between aspartic acid residues of the peroxidase having a spatial distribution that is complementary to the distribution of essential and highly conserved residues of cytochrome c. In a first attempt to test this model, carboxyl groups of cytochrome c peroxidase have been modified with a water-soluble carbodiimide, either alone or in combination with a nucleophile. Modification led to the loss of up to 90% of the ferrocytochrome c peroxidase activity. At least 4-5 carboxyl groups out of a total of 48, but none of the heme carboxyls, were modified in a derivative with 14% residual activity. In the peroxidase.cytochrome c complex the rate of peroxidase inactivation is slowed and approximately 2 carboxyl groups are protected from chemical modification. In the presence of the carbodiimide, cytochrome c and peroxidase were cross-linked to form a covalent 1:1 complex and the linkage sites were preliminarily characterized. Cross-linking occurred to carboxyl groups of the NH2-terminal fragment 1-119 and of fragment 172-229. The four crucial aspartates of the hypothetical model are located in these same two sequence regions.
Authors:
B Waldmeyer; R Bechtold; H R Bosshard; T L Poulos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  257     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1982 Jun 
Date Detail:
Created Date:  1982-07-22     Completed Date:  1982-07-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6073-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cross-Linking Reagents / pharmacology
Cytochrome c Group / metabolism*
Cytochrome-c Peroxidase / metabolism*
Electron Transport
Ethyldimethylaminopropyl Carbodiimide / pharmacology*
Horses
Kinetics
Models, Biological
Multienzyme Complexes / metabolism*
Myocardium
Peptide Fragments / analysis
Peroxidases / metabolism*
Saccharomyces cerevisiae / enzymology
Grant Support
ID/Acronym/Agency:
RR-00757/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Cross-Linking Reagents; 0/Cytochrome c Group; 0/Multienzyme Complexes; 0/Peptide Fragments; 1892-57-5/Ethyldimethylaminopropyl Carbodiimide; EC 1.11.1.-/Peroxidases; EC 1.11.1.5/Cytochrome-c Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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