Document Detail


A cytochemical and immunocytochemical analysis of the wall labyrinth apparatus in leaf transfer cells in Elodea canadensis.
MedLine Citation:
PMID:  21289025     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND AIMS: Transfer cells are plant cells specialized in apoplast/symplast transport and characterized by a distinctive wall labyrinth apparatus. The molecular architecture and biochemistry of the labyrinth apparatus are poorly known. The leaf lamina in the aquatic angiosperm Elodea canadensis consists of only two cell layers, with the abaxial cells developing as transfer cells. The present study investigated biochemical properties of wall ingrowths and associated plasmalemma in these cells.
METHODS: Leaves of Elodea were examined by light and electron microscopy and ATPase activity was localized cytochemically. Immunogold electron microscopy was employed to localize carbohydrate epitopes associated with major cell wall polysaccharides and glycoproteins.
KEY RESULTS: The plasmalemma associated with the wall labyrinth is strongly enriched in light-dependent ATPase activity. The wall ingrowths and an underlying wall layer share an LM11 epitope probably associated with glucuronoarabinoxylan and a CCRC-M7 epitope typically associated with rhamnogalacturonan I. No labelling was observed with LM10, an antibody that recognizes low-substituted and unsubstituted xylan, a polysaccharide consistently associated with secondary cell walls. The JIM5 and JIM7 epitopes, associated with homogalacturonan with different degrees of methylation, appear to be absent in the wall labyrinth but present in the rest of cell walls.
CONCLUSIONS: The wall labyrinth apparatus of leaf transfer cells in Elodea is a specialized structure with distinctive biochemical properties. The high level of light-dependent ATPase activity in the plasmalemma lining the wall labyrinth is consistent with a formerly suggested role of leaf transfer cells in enhancing inorganic carbon inflow. The wall labyrinth is a part of the primary cell wall. The discovery that the wall ingrowths in Elodea have an antibody-binding pattern divergent, in part, from that of the rest of cell wall suggests that their carbohydrate composition is modulated in relation to transfer cell functioning.
Authors:
Roberto Ligrone; Kevin C Vaughn; Nicoletta Rascio
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-02-02
Journal Detail:
Title:  Annals of botany     Volume:  107     ISSN:  1095-8290     ISO Abbreviation:  Ann. Bot.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-28     Completed Date:  2011-07-12     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0372347     Medline TA:  Ann Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  717-22     Citation Subset:  IM    
Affiliation:
Dipartimento di Scienze Ambientali, Seconda Università di Napoli, via Vivaldi 43, Caserta, Italy. roberto.ligrone@unina2.it
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MeSH Terms
Descriptor/Qualifier:
Cell Wall / metabolism*,  ultrastructure
Hydrocharitaceae / cytology*,  metabolism*,  ultrastructure
Immunohistochemistry
Plant Leaves / anatomy & histology,  cytology*,  ultrastructure
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