Document Detail


The cylindrical or tubiliform glands of Nephila clavipes.
MedLine Citation:
PMID:  3950569     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cylindrical or tubiliform glands of the spider Nephila clavipes have been studied and compared to the large ampullates on which we have previously reported. The three pairs of cylindrical or tubiliform glands secrete the fibroin for the organism's egg case. Their solubilized luminar contents migrate as a homogeneous band in Sodium dodecyl sulfate polyacrylamide gel electrophoresis and turn out to be a larger protein than that produced by the large ampullates. The excised cylindrical glands remain metabolically active for several hours in a simple culture medium, where fibroin synthesis can be monitored through the incorporation of 14C alanine. The glands' response to a fibroin production stimulus does not reach the magnitude displayed by the large ampullates, but this is to be expected since their products supply different functions in this organism. This fibroin also seems to be elongated discontinuously. Translational pauses have been detected in the secretory epithelium of cylindrical and large ampullate glands of Nephila as well as in the silk glands of Bombyx mori. Since these glands produce the fibroin for the females egg case, they should prove to be an interesting model system.
Authors:
G C Candelas; A Ortiz; C Molina
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  237     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1986 Feb 
Date Detail:
Created Date:  1986-04-16     Completed Date:  1986-04-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  281-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alanine / metabolism
Animals
Carbon Radioisotopes
Fibroins / biosynthesis*,  isolation & purification
Histidine / metabolism
Molecular Weight
Sebaceous Glands / metabolism
Spiders / metabolism*
Grant Support
ID/Acronym/Agency:
RR-08102-13/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 56-41-7/Alanine; 71-00-1/Histidine; 9007-76-5/Fibroins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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