| The cyclin-dependent kinase inhibitors, cki-1 and cki-2, act in overlapping but distinct pathways to control cell cycle quiescence during C. elegans development. | |
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MedLine Citation:
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PMID: 19597327 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cyclin-dependent kinase inhibitors (CKIs) are major contributors to the decision to enter or exit the cell cycle. The Caenorhabditis elegans genome encodes two CKIs belonging to the Cip/Kip family, cki-1 and cki-2. cki-1 has been shown to act as a canonical negative regulator of cell cycle entry, while the role of cki-2 remains unclear. We identified cki-2 in a genome-wide RNAi screen to reveal genes essential for developmental cell cycle quiescence. Examination of cki-2 knockout animals revealed extra rounds of cell divisions, verifying a role in establishing or maintaining the temporary cell cycle arrest. Despite the overlapping defects, the pathways mediated by cki-1 and cki-2 are discrete since the extra cell phenotype conferred by a putative cki-2(null) mutation is enhanced upon additional loss of cki-1 activity. Moreover, the extra cell division defect of cki-2 is not increased with the additional loss of lin-35 Rb, as is seen with cki-1. Thus, both cki-1 and cki-2 mediate cell cycle quiescence, but our genetic and phenotypic analyses demonstrate that they act within distinct pathways to exert control over the cell cycle machinery. |
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Authors:
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Sarah H Buck; Daniel Chiu; R Mako Saito |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-08-25 |
Journal Detail:
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Title: Cell cycle (Georgetown, Tex.) Volume: 8 ISSN: 1551-4005 ISO Abbreviation: Cell Cycle Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-08-17 Completed Date: 2009-10-27 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 101137841 Medline TA: Cell Cycle Country: United States |
Other Details:
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Languages: eng Pagination: 2613-20 Citation Subset: IM |
Affiliation:
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Department of Genetics, Dartmouth Medical School, Hanover, NH 03755, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / genetics, physiology* Cell Cycle / genetics, physiology* Cyclin-Dependent Kinase Inhibitor Proteins / genetics, physiology* Gene Expression Regulation, Developmental / genetics, physiology Mutation Phenotype Polymerase Chain Reaction RNA, Bacterial |
| Grant Support | |
ID/Acronym/Agency:
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GM077031/GM/NIGMS NIH HHS; R01 GM077031-02/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Caenorhabditis elegans Proteins; 0/Cyclin-Dependent Kinase Inhibitor Proteins; 0/RNA I; 0/RNA, Bacterial; 0/cki-1 protein, C elegans; 0/cki-2 protein, C elegans |
| Comments/Corrections | |
Comment In:
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Cell Cycle. 2009 Nov 1;8(21):3433-4
[PMID:
19823011
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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