Document Detail


A cyclin-dependent kinase-activating kinase regulates differentiation of root initial cells in Arabidopsis.
MedLine Citation:
PMID:  11069305     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell division and differentiation continue throughout the plant life cycle without significant loss of control. However, little is known about the mechanisms that allow the continuous development of meristems. Cell division is controlled by a family of cyclin-dependent kinases (CDKs). CDK-activating kinases (CAKs) are known to phosphorylate and activate almost all CDKs and thus may have a crucial role in controlling CDK activities in each cell of the meristems. Here, we show that overexpression of sense or antisense gene for Cak1At in Arabidopsis by using the glucocorticoid-mediated transcriptional induction system resulted in a reduction of CDK activities. After 14-24 h of glucocorticoid treatment, starch granules appeared in columellar initials in the root meristem, and cortical initials were periclinally divided into cortical and endodermal cells. Accumulation of the cyclin beta-glucuronidase fusion protein ceased after 72 h of glucocorticoid treatment. Our results indicate that a change of Cak1At activity leads to differentiation of initial cells, followed by cessation of cell division. Therefore, we propose that differentiation of initial cells is controlled by Cak1At but is maintained independent of cell division.
Authors:
M Umeda; C Umeda-Hara; H Uchimiya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  97     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-12     Completed Date:  2001-05-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  13396-400     Citation Subset:  IM    
Affiliation:
Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113-0032, Japan. mumede@imcbns.iam.u-tokyo.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / cytology,  genetics,  physiology*
Arabidopsis Proteins*
Cell Differentiation
Cell Division
Cloning, Molecular
Cyclins / genetics
DNA, Antisense / genetics
Dexamethasone / pharmacology
Gene Expression Regulation, Plant / drug effects
Genetic Vectors
Glucocorticoids / pharmacology
Glucuronidase / genetics
Meristem / cytology
Phosphorylation
Plant Roots / cytology*,  drug effects,  physiology
Plants, Genetically Modified
Protein-Serine-Threonine Kinases / genetics,  metabolism*
Recombinant Fusion Proteins / biosynthesis
Rhizobium / genetics
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Cyclins; 0/DNA, Antisense; 0/Glucocorticoids; 0/Recombinant Fusion Proteins; 50-02-2/Dexamethasone; EC 2.7.1.-/Cak1at protein, Arabidopsis; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 3.2.1.31/Glucuronidase
Comments/Corrections

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