| The cyclic nucleotide-gated calmodulin-binding channel AtCNGC10 localizes to the plasma membrane and influences numerous growth responses and starch accumulation in Arabidopsis thaliana. | |
| | |
MedLine Citation:
|
PMID: 16944199 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cyclic nucleotide gated channels (CNGCs) that are regulated by calmodulin (CaM) have been shown to play essential roles in signal transduction, metabolism, and growth in animals. By contrast, very little is known about the subcellular location and the function of these channels in plants. Here we report on the effects of antisense suppression of the expression of AtCNGC10, a putative K+ channel, and the immunolocalization of the protein using an AtCNGC10-specific antiserum. In Arabidopsis thaliana leaves, AtCNGC10 was localized to the plasma membrane of mesophyll and parenchyma cells. Antisense AtCNGC10 plants had 40% of the AtCNGC10 mRNA levels and virtually undetectable protein levels relative to wild type plants. Antisense expression of AtCNGC10 did not affect the mRNA levels of AtCNGC13, the most closely related CNGC family member in the genome. Relative to wild type Columbia, antisense AtCNGC10 plants flowered 10 days earlier, and had a 25% reduction in leaf surface area, thickness and palisade parenchyma cell length. Their roots responded more slowly to gravitropic changes and the chloroplasts accumulated more starch. We propose that AtCNGC10, through interactions with CaM and cGMP, modulates cellular K+ balance across the plasma membrane, and that perturbations of this K+ gradient affect numerous growth and developmental processes. |
| | |
Authors:
|
Tamás Borsics; David Webb; Christine Andeme-Ondzighi; L Andrew Staehelin; David A Christopher |
Related Documents
:
|
17449649 - Molecular evolution of lysin motif-type receptor-like kinases in plants. 16492479 - Plant sex pheromones. 23017909 - Osmiox, a myo-inositol oxygenase gene, improves drought tolerance through scavenging of... 23440439 - Chemical and biomarker responses for site-specific quality assessment of the lake maggi... 23677119 - Immunolocalization of iaa and aba in roots and needles of radiata pine (pinus radiata) ... 23062559 - Dissipation of difenoconazole in rice, paddy soil, and paddy water under field conditions. 546619 - Occupational contact dermatitis due to codiaeum variegatum and possibly to aeschynantus... 15296319 - Adsorption of organic vapors to air-dry soils: model predictions and experimental valid... 23297689 - Chemical composition and antibacterial activities of the essential oils isolated from j... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2006-08-31 |
Journal Detail:
|
Title: Planta Volume: 225 ISSN: 0032-0935 ISO Abbreviation: Planta Publication Date: 2007 Feb |
Date Detail:
|
Created Date: 2007-02-02 Completed Date: 2007-06-26 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 1250576 Medline TA: Planta Country: Germany |
Other Details:
|
Languages: eng Pagination: 563-73 Citation Subset: IM |
Affiliation:
|
Department of Molecular Biosciences and Bioengineering, University of Hawaii, 1955 East-West Road, Agsciences 218, Honolulu, HI 96822, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Arabidopsis
/
genetics,
growth & development,
metabolism* Arabidopsis Proteins / genetics, metabolism* Calmodulin / metabolism* Cell Membrane / metabolism, ultrastructure Chloroplasts / metabolism, ultrastructure Cyclic Nucleotide-Gated Cation Channels Gene Expression Regulation, Plant Immunoblotting Ion Channels / genetics, metabolism* Microscopy, Electron, Transmission Microscopy, Immunoelectron Plant Leaves / metabolism*, ultrastructure Protein Binding Starch / metabolism* Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Arabidopsis Proteins; 0/Calmodulin; 0/Cyclic Nucleotide-Gated Cation Channels; 0/Ion Channels; 9005-25-8/Starch |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Alpha-tocopherol may influence cellular signaling by modulating jasmonic acid levels in plants.
Next Document: Medicago truncatula syntaxin SYP132 defines the symbiosome membrane and infection droplet membrane i...