| Red/green cyanobacteriochromes: sensors of color and power. | |
| | |
MedLine Citation:
|
PMID: 23151047 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Phytochromes are red/far-red photoreceptors using cysteine-linked linear tetrapyrrole (bilin) chromophores to regulate biological responses to light. Light absorption triggers photoisomerization of the bilin between 15Z and 15E photostates. The related cyanobacteriochromes (CBCRs) extend the photosensory range of the phytochrome superfamily to shorter wavelengths of visible light. Several subfamilies of CBCRs have been described. Representatives of one such subfamily, including AnPixJ and NpR6012g4, exhibit red/green photocycles in which the 15Z photostate is red-absorbing like that of phytochrome but the 15E photoproduct is instead green-absorbing. Using recombinant expression of individual CBCR domains in Escherichia coli, we fully survey the red/green subfamily from the cyanobacterium Nostoc punctiforme. In addition to fourteen new photoswitching CBCRs, one apparently photochemically inactive protein exhibiting intense red fluorescence was observed. We describe a novel orange/green photocycle in one of these CBCRs, NpF2164g7. Dark reversion varied in this panel of CBCRs; some examples were stable as the 15E photoproduct for days, while others reverted to the 15Z dark state in minutes or even seconds. In the case of NpF2164g7, dark reversion was so rapid that reverse photoconversion of the green-absorbing photoproduct was not significant in restoring the dark state, resulting in a broadband response to light. Our results demonstrate that red/green CBCRs can thus act as sensors for the color or intensity of the ambient light environment. |
| | |
Authors:
|
Nathan Clarke Rockwell; Shelley S Martin; J Clark Lagarias |
Related Documents
:
|
22918917 - Adaptive sports ankle prosthetics. 22404697 - Effects of russian current and low-frequency pulsed current on discomfort level and cur... 23629157 - Manipulating non-innocent π-spacers: the challenges of using 2,6-disubstituted bodipy ... 3304587 - Sources and sinks of light-evoked delta [k+]o in the vertebrate retina. 9747497 - Veridical perception of global motion from disparate component motions. 17442297 - Variability of the twitch parameters of the rat medial gastrocnemius motor units--exper... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-11-14 |
Journal Detail:
|
Title: Biochemistry Volume: - ISSN: 1520-4995 ISO Abbreviation: Biochemistry Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-11-15 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Retherapy with bendamustine-containing regimens in patients with relapsed/refractory chronic lymphoc...
Next Document: Trop-2 protein overexpression is an independent marker for predicting disease recurrence in endometr...