Document Detail

A cyanase is transcriptionally regulated by arginine and involved in cyanate decomposition in Sordaria macrospora.
MedLine Citation:
PMID:  18796334     Owner:  NLM     Status:  MEDLINE    
Cyanase degrades toxic cyanate to NH3 and CO2 in a bicarbonate-dependent reaction. High concentrations of cyanate are fairly toxic to organisms. Here, we characterize a eukaryotic cyanase for the first time. We have isolated the cyn1 gene encoding a cyanase from the filamentous ascomycete Sordaria macrospora and functionally characterized the cyn1 product after heterologous expression in Escherichia coli. Site-directed mutagenesis confirmed a predicted catalytic centre of three conserved amino-acids. A Deltacyn1 knockout in S. macrospora was totally devoid of cyanase activity and showed an increased sensitivity to exogenously supplied cyanate in an arginine-depleted medium, defects in ascospore germination, but no other obvious morphological phenotype. By means of real-time PCR we have demonstrated that the transcriptional level of cyn1 is markedly elevated in the presence of cyanate and down-regulated by addition of arginine. The putative functions of cyanase in fungi are discussed.
Skander Elleuche; Stefanie Pöggeler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-29
Journal Detail:
Title:  Fungal genetics and biology : FG & B     Volume:  45     ISSN:  1096-0937     ISO Abbreviation:  Fungal Genet. Biol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-10     Completed Date:  2008-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607601     Medline TA:  Fungal Genet Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1458-69     Citation Subset:  IM    
Institute of Microbiology and Genetics, Department of Genetics of Eukaryotic Microorganisms, Georg-August University, Grisebachstr. 8, D-37077 Göttingen, Germany.
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MeSH Terms
Amino Acid Sequence
Arginine / metabolism*
Ascomycota / classification,  enzymology*,  genetics,  metabolism
Biodegradation, Environmental
Carbon-Nitrogen Lyases / chemistry,  genetics*,  metabolism
Cyanates / metabolism*
Enzyme Stability
Escherichia coli / genetics,  metabolism
Fungal Proteins / chemistry,  genetics*,  metabolism
Gene Expression Regulation, Enzymologic*
Gene Expression Regulation, Fungal
Molecular Sequence Data
Sequence Alignment
Transcription, Genetic*
Reg. No./Substance:
0/Cyanates; 0/Fungal Proteins; 74-79-3/Arginine; EC hydrolase; EC 4.3.-/Carbon-Nitrogen Lyases

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