| A cyanase is transcriptionally regulated by arginine and involved in cyanate decomposition in Sordaria macrospora. | |
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MedLine Citation:
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PMID: 18796334 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cyanase degrades toxic cyanate to NH3 and CO2 in a bicarbonate-dependent reaction. High concentrations of cyanate are fairly toxic to organisms. Here, we characterize a eukaryotic cyanase for the first time. We have isolated the cyn1 gene encoding a cyanase from the filamentous ascomycete Sordaria macrospora and functionally characterized the cyn1 product after heterologous expression in Escherichia coli. Site-directed mutagenesis confirmed a predicted catalytic centre of three conserved amino-acids. A Deltacyn1 knockout in S. macrospora was totally devoid of cyanase activity and showed an increased sensitivity to exogenously supplied cyanate in an arginine-depleted medium, defects in ascospore germination, but no other obvious morphological phenotype. By means of real-time PCR we have demonstrated that the transcriptional level of cyn1 is markedly elevated in the presence of cyanate and down-regulated by addition of arginine. The putative functions of cyanase in fungi are discussed. |
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Authors:
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Skander Elleuche; Stefanie Pöggeler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-08-29 |
Journal Detail:
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Title: Fungal genetics and biology : FG & B Volume: 45 ISSN: 1096-0937 ISO Abbreviation: Fungal Genet. Biol. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-10 Completed Date: 2008-11-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9607601 Medline TA: Fungal Genet Biol Country: United States |
Other Details:
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Languages: eng Pagination: 1458-69 Citation Subset: IM |
Affiliation:
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Institute of Microbiology and Genetics, Department of Genetics of Eukaryotic Microorganisms, Georg-August University, Grisebachstr. 8, D-37077 Göttingen, Germany. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AM773726 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Arginine / metabolism* Ascomycota / classification, enzymology*, genetics, metabolism Biodegradation, Environmental Carbon-Nitrogen Lyases / chemistry, genetics*, metabolism Cyanates / metabolism* Enzyme Stability Escherichia coli / genetics, metabolism Fungal Proteins / chemistry, genetics*, metabolism Gene Expression Regulation, Enzymologic* Gene Expression Regulation, Fungal Molecular Sequence Data Phylogeny Sequence Alignment Transcription, Genetic* |
| Chemical | |
Reg. No./Substance:
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0/Cyanates; 0/Fungal Proteins; 74-79-3/Arginine; EC 4.2.1.104/cyanate hydrolase; EC 4.3.-/Carbon-Nitrogen Lyases |
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