| A cultured human renal epithelioid cell line responsive to vasoactive intestinal peptide. | |
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MedLine Citation:
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PMID: 2161676 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A permanently transformed cell line derived from human embryo renal cortical cells (HEK293) has been investigated for the retention of renal-specific properties. The cell line is epithelioid in growth on plastic, and transmission electron microscopy demonstrates the formation of apical zonae occludentes. There is no prominent brush-border. The response of HEK293 cell adenylate cyclase is noteworthy for the response to vasoactive intestinal peptide (half-maximal activation at 0.9 nM). The HEK adenylate cyclase response to VIP is specific, with related peptides such as glucagon and secretin being ineffective. The response to VIP is competitively antagonized by the VIP receptor antagonist (4Cl-D-Phe6,Leu17)-VIP. |
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Authors:
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N L Simmons |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental physiology Volume: 75 ISSN: 0958-0670 ISO Abbreviation: Exp. Physiol. Publication Date: 1990 May |
Date Detail:
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Created Date: 1990-07-17 Completed Date: 1990-07-17 Revised Date: 2009-09-29 |
Medline Journal Info:
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Nlm Unique ID: 9002940 Medline TA: Exp Physiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 309-19 Citation Subset: IM |
Affiliation:
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Department of Physiological Sciences, School of Biomedical Science, University of Newcastle upon Tyne. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenylate Cyclase
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metabolism* Alkaline Phosphatase / metabolism Cell Line, Transformed / cytology*, enzymology, ultrastructure Cell Membrane / metabolism Cells, Cultured Epithelium / ultrastructure Glucagon / pharmacology Humans Kidney Cortex / cytology*, enzymology Potassium / metabolism Receptors, Gastrointestinal Hormone / antagonists & inhibitors Receptors, Vasoactive Intestinal Peptide Secretin / pharmacology Sodium / metabolism Sodium-Potassium-Exchanging ATPase / metabolism Vasoactive Intestinal Peptide / pharmacology, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Gastrointestinal Hormone; 0/Receptors, Vasoactive Intestinal Peptide; 102805-45-8/vasoactive intestinal peptide, 4-chloro-Phe(6)-Leu(17)-; 1393-25-5/Secretin; 37221-79-7/Vasoactive Intestinal Peptide; 7440-09-7/Potassium; 7440-23-5/Sodium; 9007-92-5/Glucagon; EC 3.1.3.1/Alkaline Phosphatase; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase; EC 4.6.1.1/Adenylate Cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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