Document Detail

A culture device demonstrates that hydrostatic pressure increases mRNA of RGS5 in neuroblastoma and CHC1-L in lymphocytic cells.
MedLine Citation:
PMID:  14504426     Owner:  NLM     Status:  MEDLINE    
Previous studies demonstrated that mechanical forces affect a wide range of cellular behaviors. These forces regulate important cellular responses in the human body and consist of gravity, hydrostatic pressure, stretch, and shear stress, which is exerted on the vascular system by the passage of blood flow. We reasoned that these forces might be significant and dynamic regulators of cellular functions within the human body. While cellular effects of stretch and shear stress have been studied particularly with endothelial cells, little is known about the effects of gravity and hydrostatic pressure to cells. To examine the direct effect of hydrostatic pressure, we developed a culture device to confer hydrostatic pressures to cells ranging from 0 to 1,000 psi. We subjected human neuroblastoma cells and rIL-2-activated lymphocytes to a constant pressure of 20 or 100 psi for 48 h and attempted to identify genes regulated by hydrostatic pressure. Genes of regulator of G-protein signaling 5 in neuroblastoma cells and CHC1-L in lymphocytes increased after exposure to hydrostatic pressure. The results demonstrated that hydrostatic pressure directly regulates the expression of specific genes in mammalian cells. Moreover, there may be some underlying mechanisms that have common effects in altered physical environments. Our in vitro culture system may provide some insight into the mechanisms through the intracellular processes affected by mechanical forces.
Yoshinobu Manome; Natsumi Saeki; Hisao Yoshinaga; Michiko Watanabe; Shuichi Mizuno
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cells, tissues, organs     Volume:  174     ISSN:  1422-6405     ISO Abbreviation:  Cells Tissues Organs (Print)     Publication Date:  2003  
Date Detail:
Created Date:  2003-09-23     Completed Date:  2004-05-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883360     Medline TA:  Cells Tissues Organs     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  155-61     Citation Subset:  IM    
Copyright Information:
Copyright 2003 S. Karger AG, Basel
Department of Microbiology, Jikei University School of Medicine, Tokyo, Japan.
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MeSH Terms
Cell Culture Techniques / instrumentation*,  methods
Cell Line, Tumor
Hydrostatic Pressure
Lymphocytes / metabolism*
Neoplasm Proteins / genetics*,  metabolism
Neuroblastoma / metabolism*
RGS Proteins / genetics*,  metabolism
RNA, Messenger / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Stress, Mechanical
Reg. No./Substance:
0/Neoplasm Proteins; 0/RCBTB2 protein, human; 0/RGS Proteins; 0/RGS6 protein, human; 0/RNA, Messenger

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