Document Detail


The crystallographic structure of the human adenosine A2A receptor in a high-affinity antagonist-bound state: implications for GPCR drug screening and design.
MedLine Citation:
PMID:  20538452     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
G-protein-coupled receptors, GPCRs, are key elements in the vertebrate signal transduction system, and constitute the majority of drug targets. Solved 10 years ago, the crystal structure of inactive state rhodopsin with covalently linked cis-retinal as an inverse agonist provided the first near-atomic view of the GPCR architecture. The inherent instability and low abundance from both natural and recombinant sources are only two factors that long hampered a similar structure elucidation of other GPCRs that have diffusible ligands such as neurotransmitters and hormones. However, in the last three years this situation has changed with the advent of structures of the human adenosine A2A receptor, avian beta1-adrenoceptor, human beta2-adrenoceptor, squid rhodopsin and activated form of bovine (rhod)opsin. In this review the structural features of the human adenosine A2A receptor and the main differences with beta-adrenoceptor and rhodopsin structures are highlighted. Furthermore, the implications of this structural information for drug screening and structure-based drug design will be discussed.
Authors:
Veli-Pekka Jaakola; Adriaan P Ijzerman
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2010-06-09
Journal Detail:
Title:  Current opinion in structural biology     Volume:  20     ISSN:  1879-033X     ISO Abbreviation:  Curr. Opin. Struct. Biol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-26     Completed Date:  2010-12-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107784     Medline TA:  Curr Opin Struct Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  401-14     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Oulu Biocenter and Department of Biochemistry, University of Oulu, Post Office Box 3000, FIN-90014 Oulu, Finland. veli-pekka.jaakola@oulu.fi
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenosine A2 Receptor Antagonists / chemistry,  metabolism*,  pharmacology*
Animals
Computational Biology
Crystallography, X-Ray
Drug Design*
Drug Evaluation, Preclinical / methods*
Humans
Receptor, Adenosine A2A / chemistry*,  metabolism*
Chemical
Reg. No./Substance:
0/Adenosine A2 Receptor Antagonists; 0/Receptor, Adenosine A2A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Shaping up nucleic acid computation.
Next Document:  Soft tissue sarcomas in the first year of life.