Document Detail

A crystalline compound (BM-ANF1) from the Indian toad (Bufo melanostictus, Schneider) skin extract, induced antiproliferation and apoptosis in leukemic and hepatoma cell line involving cell cycle proteins.
MedLine Citation:
PMID:  17692879     Owner:  NLM     Status:  MEDLINE    
In our earlier communication, it was reported that Indian toad (Bufo melanostictus) skin extract (TSE) possesses antiproliferative and apoptogenic activity in U937 and K562 cells [Giri et al., 2006. Antiproliferative, cytotoxic and apoptogenic activity of Indian toad (Bufo melanostictus, Schneider) skin extract in U937 and K562 cells. Toxicon 48 (4), 388-400]. In the present study, a compound (BM-ANF1) has been isolated from the TSE by alumina gel column chromatography, crystallized and evaluated for its antiproliferative and apoptogenic activity in U937, K562 and HepG2 cells. BM-ANF1 produced dose-dependent inhibition of U937, K562 and HepG2 cell growth. The antiproliferative activity was reflected by the MTT assay and demonstrated by the reduced expression of proliferative cell nuclear antigen (PCNA). Flow-cytometric analysis showed that BM-ANF1 arrested the cell cycle at G1 phase and enhanced annexin-V binding in U937 and K562 cells. Scanning electron microscopic and fluorescent microscopic analysis of U937 and K562 cells revealed the apoptogenic nature of the compound. Alkaline comet assay showed that BM-ANF1 produced DNA fragmentation. The dose-dependent expression of caspase 3 indicated that the apoptogenic properties of BM-ANF1 were mediated through the activation of downstream effector nucleases in the cancer cells. The increased expression of p53 and moderate expression of p21(Cip1)/p27(Kip1) due to BM-ANF1 treatment in HepG2 cells supported that the apoptogenic activity of BM-ANF1 was mediated through p53 tumor-suppressor gene expression followed by the expression of p21(Cip1) and p27(Kip1) and it was likely to be linked with cell cycle arrest at G1 phase in cancer cells. From the present study, it may be suggested that the crystalline compound, BM-ANF1, was antiproliferative and apoptogenic in human leukemic and hepatoma cells.
Antony Gomes; Biplab Giri; Labanyamoy Kole; Archita Saha; Anindita Debnath; Aparna Gomes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-30
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  50     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-01     Completed Date:  2007-12-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  835-49     Citation Subset:  IM    
Laboratory of Toxinology and Experimental Pharmacodynamics, Department of Physiology, University of Calcutta, 92, APC Road, Kolkata 700 009, India.
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MeSH Terms
Antineoplastic Agents / chemistry,  pharmacology*
Apoptosis / drug effects*
Bufonidae / physiology*
Carcinoma, Hepatocellular / drug therapy*,  metabolism,  pathology
Cell Cycle Proteins / metabolism
Cell Line, Tumor
Cell Proliferation / drug effects
Comet Assay
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Gene Expression Regulation, Neoplastic / drug effects
K562 Cells
Lymphoma, Large B-Cell, Diffuse / drug therapy*,  metabolism,  pathology
Monocytes / drug effects,  pathology
Skin / chemistry*
Tissue Extracts / chemistry,  pharmacology*
U937 Cells
Reg. No./Substance:
0/Antineoplastic Agents; 0/Cell Cycle Proteins; 0/Tissue Extracts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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