Document Detail

The crystal structure of an unusual processivity factor, herpes simplex virus UL42, bound to the C terminus of its cognate polymerase.
MedLine Citation:
PMID:  10882068     Owner:  NLM     Status:  MEDLINE    
Herpes simplex virus DNA polymerase is a heterodimer composed of a catalytic subunit, Pol, and an unusual processivity subunit, UL42, which, unlike processivity factors such as PCNA, directly binds DNA. The crystal structure of a complex of the C-terminal 36 residues of Pol bound to residues 1-319 of UL42 reveals remarkable similarities between UL42 and PCNA despite contrasting biochemical properties and lack of sequence homology. Moreover, the Pol-UL42 interaction resembles the interaction between the cell cycle regulator p21 and PCNA. The structure and previous data suggest that the UL42 monomer interacts with DNA quite differently than does multimeric toroidal PCNA. The details of the structure lead to a model for the mechanism of UL42, provide the basis for drug design, and allow modeling of other proteins that lack sequence homology with UL42 or PCNA.
H J Zuccola; D J Filman; D M Coen; J M Hogle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular cell     Volume:  5     ISSN:  1097-2765     ISO Abbreviation:  Mol. Cell     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-07-17     Completed Date:  2000-07-17     Revised Date:  2014-06-25    
Medline Journal Info:
Nlm Unique ID:  9802571     Medline TA:  Mol Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  267-78     Citation Subset:  IM    
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MeSH Terms
Antiviral Agents
DNA-Binding Proteins / chemistry*,  metabolism
DNA-Directed DNA Polymerase / chemistry*,  metabolism
Drug Design
Exodeoxyribonucleases / chemistry*,  metabolism
Models, Molecular
Peptide Fragments / chemistry
Proliferating Cell Nuclear Antigen / chemistry,  metabolism
Protein Binding
Viral Proteins / chemistry*,  metabolism
Grant Support
Reg. No./Substance:
0/Antiviral Agents; 0/DNA-Binding Proteins; 0/Peptide Fragments; 0/Proliferating Cell Nuclear Antigen; 0/Viral Proteins; EC DNA Polymerase; EC 3.1.-/Exodeoxyribonucleases; EC 3.1.11.-/DNA polymerase, Simplexvirus

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