| The crosslinked biopolymer hyaluronic acid as artificial vitreous substitute. | |
| | |
MedLine Citation:
|
PMID: 22199245 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Background:Biopolymers are promising substances in the development of a new vitreous substitute to overcome the drawbacks associated with current hydrophobic tamponade materials.Material and Methods:Different hydrogels were assembled by crosslinking hyaluronic acid either with adipic dihydrazide (ADH) by carboxylation with ń(3-dimethylaminopropyl)-ń-ethylcarbodiimide hydrochloride (EDCI) following hydrazation or by photocrosslinking with UV-light and n-vinyl-pyrrolidinone. The refractive index and rheological properties of the obtained gels were investigated. To quantify the degradation of the hydrogels over time, free hyaluronic acid was measured photometrically by means of the degradation product uronic acid. For biocompatibility testing the hydrogels were applied on top of cultured retinal pigment epithelial cells (RPE cells) and analyzed by Live/Dead®, MTT, alamar blue viability cytotoxicity assays and flow cytometry using Annexin V-FITC and propidium iodide co-staining. The in vivo biocompatibility of the hydrogels was tested in vitrectomized rabbit eyes for up to 6 weeks.Results:The synthesized hydrogels were all clear and transparent and had a refractive index similar to human vitreous. The rheological measurements suggested sufficient viscosity and elasticity for intraocular use. Quantification of the degradation products revealed only a small decay of the gels over one month. However, the ADH-crosslinked hydrogels induced mild cytotoxicity in RPE cells. The UV-crosslinked hydrogels showed no toxicity or induction of apoptosis. In vivo the UV-crosslinked biogels remained in place for the period of six weeks and electrophysiology and histology showed excellent tissue biocompatibility.Conclusion:Biopolymers based on UV-crosslinked hyaluronic acid may be promising vitreous substitutes. |
| | |
Authors:
|
C Schramm; Ms Spitzer; S Henke-Fahle; G Steinmetz; K Januschowski; P Heiduschka; J Geis-Gerstorfer; T Biedermann; Ku Bartz-Schmidt; P Szurman |
Related Documents
:
|
1827835 - Factors affecting the normal and branched-chain acyl moieties of teicoplanin components... 15560795 - Structural determination of the polar glycoglycerolipids from thermophilic bacteria mei... 16939335 - Fatty acid profile of table olives and its multivariate characterization using unsuperv... 15351685 - Sperm membrane fatty acid composition in the eastern grey kangaroo (macropus giganteus)... 3302105 - Angiotensinogen in cerebrospinal fluid corresponds chromatographically to the gamma-for... 4149515 - Specificity of the autolysin of streptococcus (diplococcus) pneumoniae. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-22 |
Journal Detail:
|
Title: Investigative ophthalmology & visual science Volume: - ISSN: 1552-5783 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-26 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Ophthalmology, Section Experimental Ophthalmic Surgery, Eberhard-Karls University Tuebingen, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nonsense-mediated decay as the molecular cause for autosomal recessive bestrophinopathy in two unrel...
Next Document: Effect of Nifedipine on Choroidal Blood Flow Regulation during Isometric Exercise.