| A critical study of amino acid incorporation into protein by isolated liver mitochondria from adult rats. | |
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MedLine Citation:
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PMID: 4963394 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Mitochondria were isolated from rat liver in a way that kept bacterial contamination at a minimum. 2. The activity of oxidative phosphorylation was unchanged under these conditions, whereas the ability of the preparations to incorporate amino acids into protein was insignificant, though it could be enhanced somewhat by the presence of EDTA. This enhancement was sensitive to ribonuclease. 3. The active time of incorporation did not exceed 15min. at 30 degrees . 4. Microsomal contamination, as measured by glucose 6-phosphatase activity, was about 5%. 5. The ability of isolated bacteria to incorporate amino acids into protein was greatly enhanced by the addition of mitochondria or heat-inactivated mitochondria. 6. A correlation was found between the growth rate of bacteria and the amino acid-incorporating activity. 7. Amino acid incorporation by combined mitochondrial-bacterial systems was inhibited by 2,4-dinitrophenol. 8. The results confirm and extend the earlier findings made in our Laboratory that isolated liver mitochondria, when free from contaminating bacteria and obtained from adult rats, are not able to catalyse the incorporation of amino acids into protein at a measurable rate. 9. The results are discussed with special emphasis on the validity of these findings. |
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Authors:
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S Sandell; H Löw; A von der Decken |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 104 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1967 Aug |
Date Detail:
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Created Date: 1967-12-13 Completed Date: 1967-12-13 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 575-85 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism* Animals Carbon Isotopes Dinitrophenols / pharmacology Edetic Acid / pharmacology Escherichia coli / metabolism Female Germ-Free Life Liver / metabolism Male Mitochondria / metabolism* Oligomycins / pharmacology Oxidative Phosphorylation* Proteins / metabolism* Rats Ribonucleases / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbon Isotopes; 0/Dinitrophenols; 0/Oligomycins; 0/Proteins; 60-00-4/Edetic Acid; EC 3.1.-/Ribonucleases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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