| A critical perspective of the use of (13)C-isotopomer analysis by GCMS and NMR as applied to cardiac metabolism. | |
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MedLine Citation:
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PMID: 14734255 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this article is to provide a guide for metabolic physiologists and bioengineers to the combined use of gas chromatography-mass spectrometry (GCMS) and nuclear magnetic resonance (NMR) in stable isotope investigations in any biological systems. Building on our past experience with these two techniques, as applied separately to the investigation of citric acid metabolism in the ex vivo perfused rat heart we initiated a collaborative study for their critical evaluation. This article, which expands on our previous work (Mol. Cel. Biol., 2003), directly compares GCMS- and NMR-determined 13C-isotopomer and flux data obtained from ex vivo rat heart perfusion studies with 13C-substrates. Overall we have found excellent agreement between the 13C-enrichments of GCMS- and NMR-determined citric acid cycle metabolites (citrate, 2-ketoglutarate, succinate and malate) and glutamate; however the unlabeled component (M) was consistently underestimated by NMR. Despite this discrepancy there was reasonably good agreement in the relative fluxes of 13C-substrates through the citric acid cycle determined by the two techniques. Nevertheless, further investigations appear necessary before maximal advantage can be taken of the complementary 13C-isotopomer and flux data of GCMS and NMR for probing the dynamics of cellular metabolism. |
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Authors:
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Christine Des Rosiers; Steven Lloyd; Blandine Comte; John C Chatham |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Metabolic engineering Volume: 6 ISSN: 1096-7176 ISO Abbreviation: Metab. Eng. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-01-21 Completed Date: 2004-09-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9815657 Medline TA: Metab Eng Country: United States |
Other Details:
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Languages: eng Pagination: 44-58 Citation Subset: IM |
Affiliation:
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The Department of Nutrition & CHUM Research Center, University of Montreal, Montreal, Que, Canada H3C 3J7. christine.des.rosiers@umontreal.ca |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Isotopes / metabolism Citric Acid Cycle* Energy Metabolism* Gas Chromatography-Mass Spectrometry Magnetic Resonance Spectroscopy Mass Spectrometry Models, Theoretical Myocardium / metabolism* Rats |
| Grant Support | |
ID/Acronym/Agency:
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5T32HL07703/HL/NHLBI NIH HHS; CA-13148/CA/NCI NIH HHS; HL48789/HL/NHLBI NIH HHS; HL67464/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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