| A cowpea mosaic virus nanoscaffold for multiplexed antibody conjugation: application as an immunoassay tracer. | |
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MedLine Citation:
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PMID: 16216488 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cowpea mosaic virus (CPMV), an icosahedral 30 nm virus, offers a uniquely programmable biological nanoscaffold. This study reports initial optimization of the simultaneous modification of two CPMV mutants with AlexaFluor 647 fluorescent dyes and either IgG proteins or antibodies at specific sites on the virus scaffold. The capacity of CPMV as a simultaneous carrier for different types of molecules was demonstrated, specifically, when applied as a tracer in direct and sandwich immunoassays. The ability to label the virus capsid with antibody and up to 60 fluorescent dyes resulted in an improved limit of detection in SEB sandwich immunoassays, when used as a tracer, relative to a mole equivalent of dye-labeled antibody. |
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Authors:
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Kim E Sapsford; Carissa M Soto; Amy Szuchmacher Blum; Anju Chatterji; Tianwei Lin; John E Johnson; Frances S Ligler; Banahalli R Ratna |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2005-10-10 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 21 ISSN: 0956-5663 ISO Abbreviation: Biosens Bioelectron Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-01-27 Completed Date: 2006-04-13 Revised Date: 2009-07-14 |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 1668-73 Citation Subset: IM |
Affiliation:
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George Mason University, Manassas, VA 20110, USA. ksap@cbmse.nrl.navy.mil |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens
/
analysis*,
immunology Biological Assay / instrumentation*, methods Biosensing Techniques / instrumentation*, methods Comovirus / immunology* Fluorescent Antibody Technique / instrumentation, methods Immunoassay / instrumentation*, methods Microarray Analysis / instrumentation*, methods Nanotechnology / instrumentation*, methods |
| Chemical | |
Reg. No./Substance:
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0/Antigens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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