Document Detail

The cotton rat model for adenovirus ocular infection: antiviral activity of cidofovir.
MedLine Citation:
PMID:  14670595     Owner:  NLM     Status:  MEDLINE    
To determine the antiviral effects of compounds against ocular adenovirus (AdV) infection, we established an animal model of AdV infection in cotton rat eyes. Cotton rat eyes were inoculated intrastromally and topically with four AdV serotypes 4, 5, 8, and 37, and treated topically with 1% HPMPC (cidofovir) eye drops twice a day. The infected corneas were extracted and homogenized, and virus titers in the cornea specimens were determined by a plaque assay. The virus titer in AdV type 5-inoculated eyes peaked on days 0 through 3 after inoculation and virus shedding was detected for 18.0+/-2.8 days. AdV 5 antigen in the infected corneas was demonstrated in the corneal epithelial cells by immunofluorescence stain. However, for AdV serotypes 4, 8, and 37, no evidence of continued virus replication in cotton rat eyes was noted. Specimens from cidofovir-treated eyes infected with AdV 5 demonstrated a statistically significant reduction in the mean virus titer (days 3-15) (P=0.028) and virus shedding duration (P=0.0014), as compared with those of the control group.
Hisatoshi Kaneko; Shuichi Mori; Osamu Suzuki; Tomohiro Iida; Shiro Shigeta; Masafumi Abe; Shigeaki Ohno; Koki Aoki; Tatsuo Suzutani
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Antiviral research     Volume:  61     ISSN:  0166-3542     ISO Abbreviation:  Antiviral Res.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2003-12-12     Completed Date:  2004-03-02     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  8109699     Medline TA:  Antiviral Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  63-6     Citation Subset:  IM    
Department of Microbiology, Fukushima Medical University School of Medicine, 1, Hikarigaoka, Fukushima 960-1295, Japan.
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MeSH Terms
Adenoviridae Infections / drug therapy*,  virology
Adenoviruses, Human / drug effects*,  growth & development
Administration, Topical
Antigens, Viral / analysis
Antiviral Agents / pharmacology,  therapeutic use*
Conjunctivitis, Viral / drug therapy
Cornea / virology
Cytosine / administration & dosage,  analogs & derivatives*,  pharmacology,  therapeutic use*
Disease Models, Animal
Drug Evaluation, Preclinical
Eye Infections, Viral / drug therapy*
Organophosphorus Compounds / administration & dosage,  pharmacology,  therapeutic use*
Viral Plaque Assay
Virus Replication / drug effects
Virus Shedding / drug effects
Reg. No./Substance:
0/Antigens, Viral; 0/Antiviral Agents; 0/Organophosphonates; 0/Organophosphorus Compounds; 71-30-7/Cytosine; JIL713Q00N/cidofovir

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