| The cotton rat model for adenovirus ocular infection: antiviral activity of cidofovir. | |
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MedLine Citation:
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PMID: 14670595 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine the antiviral effects of compounds against ocular adenovirus (AdV) infection, we established an animal model of AdV infection in cotton rat eyes. Cotton rat eyes were inoculated intrastromally and topically with four AdV serotypes 4, 5, 8, and 37, and treated topically with 1% HPMPC (cidofovir) eye drops twice a day. The infected corneas were extracted and homogenized, and virus titers in the cornea specimens were determined by a plaque assay. The virus titer in AdV type 5-inoculated eyes peaked on days 0 through 3 after inoculation and virus shedding was detected for 18.0+/-2.8 days. AdV 5 antigen in the infected corneas was demonstrated in the corneal epithelial cells by immunofluorescence stain. However, for AdV serotypes 4, 8, and 37, no evidence of continued virus replication in cotton rat eyes was noted. Specimens from cidofovir-treated eyes infected with AdV 5 demonstrated a statistically significant reduction in the mean virus titer (days 3-15) (P=0.028) and virus shedding duration (P=0.0014), as compared with those of the control group. |
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Authors:
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Hisatoshi Kaneko; Shuichi Mori; Osamu Suzuki; Tomohiro Iida; Shiro Shigeta; Masafumi Abe; Shigeaki Ohno; Koki Aoki; Tatsuo Suzutani |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Antiviral research Volume: 61 ISSN: 0166-3542 ISO Abbreviation: Antiviral Res. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2003-12-12 Completed Date: 2004-03-02 Revised Date: 2013-05-30 |
Medline Journal Info:
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Nlm Unique ID: 8109699 Medline TA: Antiviral Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 63-6 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Fukushima Medical University School of Medicine, 1, Hikarigaoka, Fukushima 960-1295, Japan. h-kane@chive.ocn.ne.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae Infections
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drug therapy*,
virology Adenoviruses, Human / drug effects*, growth & development Administration, Topical Animals Antigens, Viral / analysis Antiviral Agents / pharmacology, therapeutic use* Conjunctivitis, Viral / drug therapy Cornea / virology Cytosine / administration & dosage, analogs & derivatives*, pharmacology, therapeutic use* Disease Models, Animal Drug Evaluation, Preclinical Eye Infections, Viral / drug therapy* Female Organophosphonates* Organophosphorus Compounds / administration & dosage, pharmacology, therapeutic use* Rats Sigmodontinae Viral Plaque Assay Virus Replication / drug effects Virus Shedding / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Viral; 0/Antiviral Agents; 0/Organophosphonates; 0/Organophosphorus Compounds; 71-30-7/Cytosine; JIL713Q00N/cidofovir |
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