| Sodium/glucose cotransporter-1, sweet receptor, and disaccharidase expression in the intestine of the domestic dog and cat: two species of different dietary habit. | |
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MedLine Citation:
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PMID: 20980625 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The domestic cat (Felis catus), a carnivore, naturally eats a very low carbohydrate diet. In contrast, the dog (Canis familiaris), a carno-omnivore, has a varied diet. This study was performed to determine the expression of the intestinal brush border membrane sodium/glucose cotransporter, SGLT1, sweet receptor, T1R2/T1R3, and disaccharidases in these species adapted to contrasting diets. The expression (this includes function) of SGLT1, sucrase, maltase and lactase were determined using purified brush border membrane vesicles and by quantitative immunohistochemistry of fixed tissues. The pattern of expression of subunits of the sweet receptor T1R2 and T1R3 was assessed using fluorescent immunohistochemistry. In proximal, middle, and distal small intestine, SGLT1 function in dogs was 1.9- to 2.3-fold higher than in cats (P = 0.037, P = 0.0011, P = 0.027, respectively), and SGLT1 protein abundance followed an identical pattern. Both cats and dogs express T1R3 in a subset of intestinal epithelial cells, and dogs, but not cats, express T1R2. In proximal and middle regions, there were 3.1- and 1.6-fold higher lactase (P = 0.006 and P = 0.019), 4.4- and 2.9-fold higher sucrase (both P < 0.0001), and 4.6- and 3.1-fold higher maltase activity (P = 0.0026 and P = 0.0005), respectively, in the intestine of dogs compared with cats. Dogs have a potential higher capacity to digest and absorb carbohydrates than cats. Cats may suffer from carbohydrate malabsorption following ingestion of high-carbohydrate meals. However, dogs have a digestive ability to cope with diets containing significant levels of carbohydrate. |
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Authors:
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D J Batchelor; M Al-Rammahi; A W Moran; J G Brand; X Li; M Haskins; A J German; S P Shirazi-Beechey |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-10-27 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 300 ISSN: 1522-1490 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-24 Completed Date: 2011-01-20 Revised Date: 2012-01-02 |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R67-75 Citation Subset: IM |
Affiliation:
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Department of Veterinary Preclinical Sciences, University of Liverpool, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Carbohydrate Metabolism / physiology Cats / metabolism* Diet* Disaccharidases / metabolism* Dogs / metabolism* Female Glucose / metabolism Intestines / metabolism* Male Microvilli / metabolism Molecular Sequence Data Receptors, Cell Surface / metabolism* Sodium-Glucose Transporter 1 / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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PR02512/PR/OCPHP CDC HHS; //Biotechnology and Biological Sciences Research Council |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Cell Surface; 0/Sodium-Glucose Transporter 1; 50-99-7/Glucose; EC 3.2.1.-/Disaccharidases |
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