Document Detail

The correlation between the regulation of recombinant human IGF-2 on eye growth and form-deprivation in guinea pig.
MedLine Citation:
PMID:  20135141     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: By investigating the effects of recombinant human IGF-2 (rhIGF-2) on the diopter, axial eye length and the expression of IGF-2 in non-form-deprivation (FD) and FD eyes of guinea pig, we tried to elucidate the relationship between the effects of rhIGF-2 on eye growth and FD in guinea pig. METHODS: Eighty 3-week-old guinea pigs were included in the study, which were divided into two groups randomly. Group A (n = 40) was the non-FD group, Thirty-two guinea pigs received intravitreal injections of either 1 ng, 10 ng, 100 ng rhIGF-2 or bovine serum albumin (BSA) to the right eye; eight guinea pigs who received no intravitreal injections served as control. Group B (n = 40) was the FD group; the right eyes were form-deprived, and received the same disposals as group A. The diopter and the axial eye length of all guinea pigs were measured at day 14. The expression of IGF-2 in the retina was measured by Western blot. RESULTS: After 14 days, FD eyes were high myopia. The axial length of FD eyes was significant for longer than that of non-FD eyes. The expression of IGF-2 in the retina of FD eyes was up-regulated. In non-FD group, there was no significant difference in the diopter and axial eye length among rhIGF-2 injection eyes, BSA injection eyes and control eyes, but the expression of IGF-2 in the retina of 10 ng and 100 ng rhIGF-2 injection eyes was obviously down-regulated. In the FDM (form-deprivation myopia) group, the myopic diopter and axial eye length increased in 10 ng and 100 ng rhIGF-2 injection eyes compared with those in BSA injection eyes and control eyes. The level of IGF-2 expression in the retina of 10 ng and 100 ng rhIGF-2 injection eyes was obviously down-regulated. The diopter and expression of IGF-2 in the retina of rhIGF-2 injection eyes was negatively correlated with the dose of each injection, which was positively correlated with the axial length. CONCLUSION: RhIGF-2 intravitreous injection does not affect the diopter and axial length in non-FD eyes of guinea pig, while it can induce a significant increase in myopic diopter and axial length of FD eyes. RhIGF-2 can promote the development of FDM on the condition of FD.
Zhi-hong Deng; Jia Tan; Shuang-zhen Liu; Shao-zhen Zhao; Jian-tao Wang
Publication Detail:
Type:  Journal Article     Date:  2010-02-05
Journal Detail:
Title:  Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv f?r klinische und experimentelle Ophthalmologie     Volume:  248     ISSN:  1435-702X     ISO Abbreviation:  Graefes Arch. Clin. Exp. Ophthalmol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-02     Completed Date:  2010-05-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205248     Medline TA:  Graefes Arch Clin Exp Ophthalmol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  519-25     Citation Subset:  IM    
Eye Center, Tianjin Medical University, Tianjin, 300070, People's Republic of China.
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MeSH Terms
Blotting, Western
Eye / growth & development*
Guinea Pigs
Insulin-Like Growth Factor II / administration & dosage,  metabolism*
Myopia / etiology,  metabolism*
Recombinant Proteins / administration & dosage,  metabolism*
Retina / metabolism*
Sensory Deprivation
Vitreous Body
Reg. No./Substance:
0/Recombinant Proteins; 67763-97-7/Insulin-Like Growth Factor II

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