| A correlated stereological and functional studies on the long-term effects of ACTH on rat adrenal cortex. | |
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MedLine Citation:
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PMID: 3030828 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the study was to investigate the effect of prolonged ACTH administration on quantitative structural changes of the rat adrenal cortex and on function of its cells with particular emphasis on correlation of the results of biochemical estimations with stereologic parameters. Daily injections of 20 micrograms ACTH (Synacthen, Depot) for 35 days results in a marked enlargement of the cortex due to an increase in the volume of all the zones. This increase depends upon hypertrophy and hyperplasia of parenchymal cells. At day 21 of experiment the number of parenchymal cells markedly decreased if compared with day 14, the lost of cells being observed mainly in the zona reticularis. Prolonged ACTH treatment only insignificantly changed serum corticosterone concentration and--if calculated per mg of adrenal weight--did not change adrenal corticosterone concentration and 11 beta-hydroxylase activity and decreased corticosterone output by adrenal homogenate. If expressed per adrenocortical cell or per pair of glands, ACTH increased corticosterone concentration and 11 beta-hydroxylase activity while the drop in corticosterone output occurred only at days 28 and 35 of experiment. The striking differences in and among various functional parameters depicting adrenal steroidogenesis show for necessity--in case of long-term experiments leading to hypertrophy or atrophy of the gland--of using coupled stereologic and biochemical techniques which better evaluate the cytophysiological state of adrenocortical cells. |
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Authors:
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K Malendowicz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society Volume: 24 ISSN: 0239-8508 ISO Abbreviation: Folia Histochem. Cytobiol. Publication Date: 1986 |
Date Detail:
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Created Date: 1987-04-27 Completed Date: 1987-04-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8502651 Medline TA: Folia Histochem Cytobiol Country: POLAND |
Other Details:
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Languages: eng Pagination: 203-11 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Cortex
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anatomy & histology,
drug effects*,
metabolism Adrenocorticotropic Hormone / pharmacology* Animals Corticosterone / metabolism Female Organ Size / drug effects Rats Rats, Inbred Strains Steroid 11-beta-Hydroxylase / metabolism |
| Chemical | |
Reg. No./Substance:
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50-22-6/Corticosterone; 9002-60-2/Adrenocorticotropic Hormone; EC 1.14.15.4/Steroid 11-beta-Hydroxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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