| A convenient and efficient method for the extraction and fractionation of steroid hormones from serum or urine. | |
| | |
MedLine Citation:
|
PMID: 7217897 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A convenient solid phase method for extracting and fractionating steroid hormones from serum or urine is presented. The influence of the solvent used for extraction, of the body fluid to be extracted, and of the degree of sample dilution on the efficiency of extraction was studied for progesterone and cortisol. The potency of fractionation was demonstrated by the separation of the steroid pairs, deoxycortisol--cortisol and oestradiol--oestriol, from a urine sample. Influence of ionic strength, pH and temperature on the reproducibility was assessed for deoxycortisol in undiluted urine. With respect to practicability and efficiency, this method has proved to be considerably superior to conventional liquid-liquid techniques. |
| | |
Authors:
|
M Schöneshöfer; A Fenner |
Related Documents
:
|
1052597 - Spontaneous conversions to aldosterone. 18547597 - Inclusion complex-based solid-phase extraction of steroidal compounds with entrapped be... 15195467 - Development of an analytical method for 3-monochloropropane-1,2-diol in soy sauce using... 17889887 - Development and validation of a method for simultaneous analysis of the boar taint comp... 4295867 - Characterization of vitamin k from bovine liver. 893577 - Gas chromatographic and gas chromatographic-mass spectrometric analysis of ampicillin. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of clinical chemistry and clinical biochemistry. Zeitschrift für klinische Chemie und klinische Biochemie Volume: 19 ISSN: 0340-076X ISO Abbreviation: J. Clin. Chem. Clin. Biochem. Publication Date: 1981 Feb |
Date Detail:
|
Created Date: 1981-06-13 Completed Date: 1981-06-13 Revised Date: 2007-07-24 |
Medline Journal Info:
|
Nlm Unique ID: 7701860 Medline TA: J Clin Chem Clin Biochem Country: GERMANY, WEST |
Other Details:
|
Languages: eng Pagination: 71-4 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
17-Hydroxycorticosteroids
/
isolation & purification* Chemical Fractionation Cortodoxone / isolation & purification* Estriol / isolation & purification* Humans Hydrocortisone / isolation & purification* Methods Progesterone / isolation & purification* Solvents |
| Chemical | |
Reg. No./Substance:
|
0/17-Hydroxycorticosteroids; 0/Solvents; 152-58-9/Cortodoxone; 50-23-7/Hydrocortisone; 50-27-1/Estriol; 57-83-0/Progesterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation and identification of 2-oxo-5-guanidinovaleric acid in urine of patients with hyperarginin...
Next Document: Sensitivity of several commercial thromboplastins to non-carboxylated clotting factors II and X (aut...