Document Detail

A convenient and efficient method for the extraction and fractionation of steroid hormones from serum or urine.
MedLine Citation:
PMID:  7217897     Owner:  NLM     Status:  MEDLINE    
A convenient solid phase method for extracting and fractionating steroid hormones from serum or urine is presented. The influence of the solvent used for extraction, of the body fluid to be extracted, and of the degree of sample dilution on the efficiency of extraction was studied for progesterone and cortisol. The potency of fractionation was demonstrated by the separation of the steroid pairs, deoxycortisol--cortisol and oestradiol--oestriol, from a urine sample. Influence of ionic strength, pH and temperature on the reproducibility was assessed for deoxycortisol in undiluted urine. With respect to practicability and efficiency, this method has proved to be considerably superior to conventional liquid-liquid techniques.
M Schöneshöfer; A Fenner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical chemistry and clinical biochemistry. Zeitschrift für klinische Chemie und klinische Biochemie     Volume:  19     ISSN:  0340-076X     ISO Abbreviation:  J. Clin. Chem. Clin. Biochem.     Publication Date:  1981 Feb 
Date Detail:
Created Date:  1981-06-13     Completed Date:  1981-06-13     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  7701860     Medline TA:  J Clin Chem Clin Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  71-4     Citation Subset:  IM    
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MeSH Terms
17-Hydroxycorticosteroids / isolation & purification*
Chemical Fractionation
Cortodoxone / isolation & purification*
Estriol / isolation & purification*
Hydrocortisone / isolation & purification*
Progesterone / isolation & purification*
Reg. No./Substance:
0/17-Hydroxycorticosteroids; 0/Solvents; 152-58-9/Cortodoxone; 50-23-7/Hydrocortisone; 50-27-1/Estriol; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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